Detailed information of TA system TA234899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 120283..120860
• Replicon: plasmid unnamed2
• Accession: NZ_CP091856
• Organism: Rhodococcus erythropolis strain CERE8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A069J573
• Locus tag: ITJ47_RS00565
• Protein ID: WP_007733685.1
• Coordinates: 120561..120860 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: ITJ47_RS00560
• Protein ID: WP_230587717.1
• Coordinates: 120283..120561 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ITJ47_RS00540 (ITJ47_00540)	116459..117241	+	783	WP_007733691.1	ParA family protein	-
ITJ47_RS00545 (ITJ47_00545)	117400..117552	+	153	WP_007733689.1	hypothetical protein	-
ITJ47_RS00550 (ITJ47_00550)	118183..119157	+	975	WP_030537938.1	DUF2637 domain-containing protein	-
ITJ47_RS00555 (ITJ47_00555)	119267..119863	+	597	WP_230587716.1	TetR family transcriptional regulator	-
ITJ47_RS00560 (ITJ47_00560)	120283..120561	+	279	WP_230587717.1	ribbon-helix-helix domain-containing protein	Antitoxin
ITJ47_RS00565 (ITJ47_00565)	120561..120860	+	300	WP_007733685.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ITJ47_RS00570 (ITJ47_00570)	120889..122739	+	1851	WP_149357466.1	hypothetical protein	-
ITJ47_RS00575 (ITJ47_00575)	122739..123536	+	798	WP_030538012.1	hypothetical protein	-
ITJ47_RS00580 (ITJ47_00580)	123709..123816	+	108	Protein_117	ArsR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..158679	158679

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10471.13 Da
• Isoelectric Point: 5.0079

>T234899 WP_007733685.1 NZ_CP091856:120561-120860 [Rhodococcus erythropolis]
MRPIHEAKLDTVRPVLVLTREMVRPHLGTVTVAPITGEIRGLSTEVSVSVANGLSADAVVSCDHITTIPVSALGNIIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T234899 NZ_LR134117:c1717721-1717371 [Serratia odorifera]
ATGTGGACAATCGTTACCCGGCCGCGGTTTGATCAATGGTTTTACCGACAAACGGATCGCGTGCAGGAAGAAATGTTAGC
CGTGTTGACCCTACTACAGCAGGATGGCCCCAATCTCGGACGTCCTCAGGTTGATACGCTGAAGGGCTCCGCCTACGGGA
ATATGAAGGAACTGCGGGTACAGACGGCAGGCCATCCCATACGTGCATGTTTTGCTTTTGATCCCCACAGGCAGGCGATC
GTTTTATGTGCCGCTGACAAAAAAGGCATGGGGCGACGCTTCTATCAGCAGTTGATAAAAACGGCGATGCCGAGTATGCC
GCCCATCTGCAAAACTCTGGAGAATGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10494.91 Da
• Isoelectric Point: 8.8578

>AT234899 WP_230587717.1 NZ_CP091856:120283-120561 [Rhodococcus erythropolis]
MVVPKRIPLRYHQHMSTQIAVRLPDEIVEFLDDEVREHRAASRASLVLRALERERRRQIAARDAEILSAATSEHDLSPLA
HHAVNRQPTGST

Nucleotide

• Length: 279 bp

>AT234899 NZ_LR134117:c1717371-1717072 [Serratia odorifera]
ATGGCAACCTTGGAACACCTGTTAGCGCAACGCTCCGCTGAAAGTCGTGAGAGAATCGCGGCCCGGGCCGATGAGATTCG
TCAGGAAATTGCCTTGGCGAAAATACGCGAAGAATTGAATATATCGCAGAAACAACTGGCTGACGCCATGGGGATTAAAC
AGCCCGCCGTCGCGAAGATGGAAAAAGTGGACAACGACCCGAAACTGTCGACATTGAAACGTTATGTCAAAGCGCTTGGC
GGAGAGCTGTCTCTCGACGTAACGTTACCGGACGGCAAACGCATCGCATTGCATCTTTGA

Structures

Toxin

• AlphaFold DB: A0A069J573