Detailed information of TA system TA234896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 54867..55603
• Replicon: plasmid pNB05-2
• Accession: NZ_CP091848
• Organism: Klebsiella pneumoniae strain NB05

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: L7D54_RS28055
• Protein ID: WP_003026803.1
• Coordinates: 55121..55603 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: L7D54_RS28050
• Protein ID: WP_003026799.1
• Coordinates: 54867..55133 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7D54_RS28005	50929..51291	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
L7D54_RS28010	51341..51691	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
L7D54_RS28015	52049..52318	+	270	WP_004152102.1	hypothetical protein	-
L7D54_RS28020	52306..52881	+	576	WP_004152103.1	hypothetical protein	-
L7D54_RS28025	52912..53406	+	495	WP_004152104.1	DNA-binding protein	-
L7D54_RS28030	53450..53818	+	369	WP_004152105.1	hypothetical protein	-
L7D54_RS28035	53852..54055	+	204	WP_004152106.1	HHA domain-containing protein	-
L7D54_RS28040	54104..54361	+	258	WP_004152107.1	hypothetical protein	-
L7D54_RS28045	54437..54691	+	255	WP_004152108.1	hypothetical protein	-
L7D54_RS28050	54867..55133	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
L7D54_RS28055	55121..55603	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
L7D54_RS28060	55804..57207	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
L7D54_RS28065	57236..57868	-	633	WP_001567369.1	hypothetical protein	-
L7D54_RS28070	58098..59444	+	1347	WP_238050200.1	ISNCY family transposase	-
L7D54_RS29195	59605..59736	+	132	WP_004218042.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / mph(A)	-	1..171032	171032

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T234896 WP_003026803.1 NZ_CP091848:55121-55603 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T234896 NZ_LR134117:115515-115931 [Serratia odorifera]
ATGAAGAAAACCTATATGCTGGACACCAATATCTGCTCTTTTATTATGCGTGAACAGCCGGAGGCGGTTATTCATCGGTT
ACAGCAATGTGCGCTGCGGAATAATCGTATCGTGGTATCAGCCATTACCTACGCCGAAATGCGCTTTGGTGCTATCGGCA
AGAAAGCCTCACCACGCCATGGCCTGCTGGTCGACGCATTTTGTCAGCGTCTCGATGCCGTATTACCCTGGGATCGCGCG
GCGGTAGACGCCACCACGACGGTCAGACAGGCGCTGGCCGCCGCCGGTACACCCATTGGCGGCAACGATGCGGCCATTGC
CGGTCATGCTATTTCAACCGACGCCATCCTGGTTACCAACAATGTCAGGGAGTTTGAGCGGGTGCCTACATTGCTCTGGG
AAGACTGGGTAAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT234896 WP_003026799.1 NZ_CP091848:54867-55133 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT234896 NZ_LR134117:115291-115518 [Serratia odorifera]
ATGAGAACCGTCGCCATTTTTAAGAACGGCAGTAACCGGGCCATTCGTCTGCCACGGGATATGGATTTTGATGGGGTGAA
TGAACTAGAAATTGTTCGGGAAGGGGATACGCTTATTTTACGGCCGGTCAAACCCAGCTGGAATTCTTTTTTGTCGGAAG
AAAACGCGGACAGCGACTTTATGGTTGAACGCCCCGATATTATTGAGGAAGGGCGCGTCAGGTTATGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66