Detailed information of TA system TA234895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 17211..17733
• Replicon: plasmid pNB05-MDM-1
• Accession: NZ_CP091847
• Organism: Klebsiella pneumoniae strain NB05

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A7H0EVH6
• Locus tag: L7D54_RS26025
• Protein ID: WP_014839901.1
• Coordinates: 17449..17733 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: L7D54_RS26020
• Protein ID: WP_014839900.1
• Coordinates: 17211..17459 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7D54_RS25995	12389..12745	+	357	WP_238050194.1	hypothetical protein	-
L7D54_RS26000	13119..13517	+	399	WP_014839899.1	H-NS family nucleoid-associated regulatory protein	-
L7D54_RS26005	13654..14262	+	609	WP_024266306.1	DUF2913 family protein	-
L7D54_RS26010	14806..15438	+	633	WP_001567369.1	hypothetical protein	-
L7D54_RS26015	15467..16870	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
L7D54_RS26020	17211..17459	+	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
L7D54_RS26025	17449..17733	+	285	WP_014839901.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L7D54_RS26030	17924..18400	+	477	WP_042934526.1	hypothetical protein	-
L7D54_RS26035	18406..19083	+	678	WP_042934501.1	hypothetical protein	-
L7D54_RS29185	19100..19819	-	720	WP_042934502.1	cation:proton antiporter	-
L7D54_RS26050	20060..20662	-	603	WP_014839902.1	HAD domain-containing protein	-
L7D54_RS26055	20659..21180	-	522	WP_048336588.1	hypothetical protein	-
L7D54_RS26060	21261..22205	-	945	WP_014839903.1	DsbA family protein	-
L7D54_RS26065	22268..22477	-	210	WP_125316032.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA5 / dfrA1 / blaNDM-1 / fosA3 / blaSHV-12 / qnrB4 / blaDHA-1 / qnrB52 / mph(A)	htpB	1..349120	349120
	-	flank	IS/Tn	-	-	15467..16870	1403

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10866.46 Da
• Isoelectric Point: 10.5831

>T234895 WP_014839901.1 NZ_CP091847:17449-17733 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDIYNATRHRN

Nucleotide

• Length: 285 bp

>T234895 NZ_LR134117:113142-113429 [Serratia odorifera]
ATGACTTATAAGATTGACTTTGACAATGCAGCCTTCAAAGAGTGGAAAAAATTAGGTGAGACTGTACGTCAACAGTTTAA
AAAGAAGCTGGCGCAGATTGTTGAGGCACCACACATTGAGGCTAACCGACTGCACTCCTTACCTGATTGCTATAAGATCA
AGCTCCGCGCATCCGGTTATCGGCTAGTCTACAAGGTCGAGGATGAAACTGTTGTGATCCTCGTCCTTGCTGTAGGTAAA
CGCGAACGGCTGGAAGTTTACAAAGCAGCCGCCACAAGGTTGGGTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT234895 WP_014839900.1 NZ_CP091847:17211-17459 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT234895 NZ_LR134117:112910-113152 [Serratia odorifera]
ATGGGTACAATGAATATTCGTATCGACGATGACTTAAAGGCCCGGGCCGGTGTAGCGCTAGACGGATTGGGGATCACAGC
CTCGGAAGCTGTACGTATGGTACTTGAATACGTTGCACAAAATAACCGCCTTCCGGTTCAGGTGGCATTACTGGCTGATG
AAGACGCAGATATCATCGAGTTAGCGCGTGAACGTCTGGCGAATCCTGGCAAACGGATCCGAGTAACACTCGATGACTTA
TAA

Structures

Toxin

• AlphaFold DB: A0A7H0EVH6

Antitoxin

• AlphaFold DB: A0A7H0EVH5