Detailed information of TA system TA023489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 148894..149574
• Replicon: chromosome
• Accession: NC_011894
• Organism: Methylobacterium nodulans ORS 2060

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: MNOD_RS42535
• Protein ID: WP_015926896.1
• Coordinates: 148894..149103 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B8IUD9
• Locus tag: MNOD_RS00645
• Protein ID: WP_015926897.1
• Coordinates: 149158..149574 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNOD_RS00635	145840..147588	+	1749	WP_015926894.1	cytochrome b/b6 domain-containing protein	-
MNOD_RS00640	147609..148652	+	1044	WP_015926895.1	2Fe-2S iron-sulfur cluster binding domain-containing protein	-
MNOD_RS42535	148894..149103	+	210	WP_015926896.1	type II toxin-antitoxin system HicA family toxin	Toxin
MNOD_RS00645	149158..149574	+	417	WP_015926897.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
MNOD_RS00650	149680..150477	-	798	WP_015926898.1	substrate-binding domain-containing protein	-
MNOD_RS00655	150626..151777	-	1152	WP_050783475.1	osmoprotectant NAGGN system M42 family peptidase	-
MNOD_RS00660	151828..153537	-	1710	WP_015926900.1	N-acetylglutaminylglutamine synthetase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (11-67)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (9-126)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 8097.17 Da
• Isoelectric Point: 10.5348

>T23489 WP_015926896.1 NC_011894:148894-149103 [Methylobacterium nodulans ORS 2060]
MSGRKRSRKTRDVIAALFKDDWELYRKGPGDHIQFKHQNKLGKVTVDNGEPEIPTGTLRNIYRQAGWEW

Nucleotide

• Length: 210 bp

>T23489 NC_011894:148894-149103 [Methylobacterium nodulans ORS 2060]
ATGTCGGGTCGCAAACGTAGTCGGAAGACACGCGATGTAATTGCCGCCCTGTTCAAAGACGATTGGGAATTATATCGCAA
AGGCCCGGGCGATCATATTCAATTCAAGCATCAGAATAAGCTCGGCAAGGTTACTGTTGACAACGGCGAGCCGGAAATCC
CCACAGGGACGCTTCGTAACATCTACAGGCAGGCCGGCTGGGAGTGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14825.82 Da
• Isoelectric Point: 4.3492

>AT23489 WP_015926897.1 NC_011894:149158-149574 [Methylobacterium nodulans ORS 2060]
MARIVMLIHEEGGVYGASFPDFPGATTVAGDLDTLYTKAAEMLAFHVEGMVEDGDEIPVPRTLQELREDPVFREDAEGAI
IGLVEVELPGKAVRVNISIEESILRRIDRAAETAGETRSGFLVAAAKARLAQTSGVSR

Nucleotide

• Length: 417 bp

>AT23489 NC_011894:149158-149574 [Methylobacterium nodulans ORS 2060]
ATGGCCCGTATCGTTATGCTGATTCACGAGGAAGGGGGCGTCTACGGCGCCTCCTTTCCTGACTTCCCCGGCGCGACGAC
CGTCGCGGGAGATCTGGACACGCTCTACACCAAGGCCGCCGAGATGCTGGCCTTCCACGTCGAGGGCATGGTCGAGGACG
GGGACGAGATCCCGGTCCCGCGCACCCTGCAGGAGCTCCGGGAGGACCCTGTCTTCCGGGAAGATGCCGAGGGCGCAATC
ATCGGCCTCGTGGAGGTCGAGCTGCCCGGCAAGGCGGTGCGCGTGAACATCTCCATTGAGGAGAGCATCCTACGCCGCAT
CGACCGCGCCGCCGAGACGGCGGGCGAGACCCGCTCGGGCTTCCTCGTTGCCGCCGCGAAGGCACGTCTCGCGCAGACCA
GCGGCGTCAGCCGGTAG

Structures

Antitoxin

• AlphaFold DB: B8IUD9