Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | phd-doc/Doc-Phd |
| Location | 49116..49717 | Replicon | plasmid pHX138.261ft_1 |
| Accession | NZ_CP091840 | ||
| Organism | Escherichia coli strain SWYHX138.261ft | ||
Toxin (Protein)
| Gene name | doc | Uniprot ID | A0A5U0JPI4 |
| Locus tag | L7H22_RS22465 | Protein ID | WP_024166788.1 |
| Coordinates | 49337..49717 (+) | Length | 127 a.a. |
Antitoxin (Protein)
| Gene name | phd | Uniprot ID | U9YQH9 |
| Locus tag | L7H22_RS22460 | Protein ID | WP_001190712.1 |
| Coordinates | 49116..49337 (+) | Length | 74 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| L7H22_RS22425 (L7H22_22425) | 44714..46129 | - | 1416 | WP_238094660.1 | undecaprenyl-phosphate glucose phosphotransferase | - |
| L7H22_RS22430 (L7H22_22430) | 46541..47509 | - | 969 | WP_074194686.1 | IS5 family transposase | - |
| L7H22_RS22435 (L7H22_22435) | 47468..47665 | + | 198 | WP_048659784.1 | DUF4752 family protein | - |
| L7H22_RS22440 (L7H22_22440) | 47662..47952 | + | 291 | WP_048659782.1 | hypothetical protein | - |
| L7H22_RS22445 (L7H22_22445) | 48022..48288 | + | 267 | WP_000946114.1 | hypothetical protein | - |
| L7H22_RS22450 (L7H22_22450) | 48291..48539 | + | 249 | WP_001286530.1 | DNA polymerase III subunit theta | - |
| L7H22_RS22455 (L7H22_22455) | 48651..49043 | + | 393 | WP_000506718.1 | S24 family peptidase | - |
| L7H22_RS22460 (L7H22_22460) | 49116..49337 | + | 222 | WP_001190712.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| L7H22_RS22465 (L7H22_22465) | 49337..49717 | + | 381 | WP_024166788.1 | type II toxin-antitoxin system death-on-curing family toxin | Toxin |
| L7H22_RS22470 (L7H22_22470) | 49801..50184 | - | 384 | Protein_53 | hypothetical protein | - |
| L7H22_RS22475 (L7H22_22475) | 50237..51463 | - | 1227 | WP_238094661.1 | transposase | - |
| L7H22_RS22480 (L7H22_22480) | 51522..51926 | + | 405 | WP_238094653.1 | IS200/IS605 family transposase | - |
| L7H22_RS22485 (L7H22_22485) | 51943..52419 | - | 477 | WP_238094654.1 | hypothetical protein | - |
| L7H22_RS22490 (L7H22_22490) | 52433..52927 | - | 495 | WP_048659776.1 | dUTP diphosphatase | - |
| L7H22_RS22495 (L7H22_22495) | 52920..53411 | - | 492 | WP_238094655.1 | hypothetical protein | - |
| L7H22_RS22500 (L7H22_22500) | 53408..53650 | - | 243 | WP_238094656.1 | hypothetical protein | - |
| L7H22_RS22505 (L7H22_22505) | 53692..54389 | + | 698 | WP_095033700.1 | IS1-like element IS1B family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(A) | galE | 1..90138 | 90138 | |
| - | inside | IScluster/Tn | - | - | 46541..64004 | 17463 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 127 a.a. Molecular weight: 13663.58 Da Isoelectric Point: 5.6406
>T234877 WP_024166788.1 NZ_CP091840:49337-49717 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI
Download Length: 381 bp
>T234877 NZ_LR134105:183231-183497 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA
Antitoxin
Download Length: 74 a.a. Molecular weight: 8133.10 Da Isoelectric Point: 4.7708
>AT234877 WP_001190712.1 NZ_CP091840:49116-49337 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR
Download Length: 222 bp
>AT234877 NZ_LR134105:182968-183231 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5U0JPI4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A829CJB6 |