Detailed information of TA system TA234876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 12429..12991
• Replicon: plasmid pHX138.261ft_1
• Accession: NZ_CP091840
• Organism: Escherichia coli strain SWYHX138.261ft

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: L7H22_RS22265
• Protein ID: WP_223232864.1
• Coordinates: 12429..12677 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: L7H22_RS22270
• Protein ID: WP_023338085.1
• Coordinates: 12722..12991 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7H22_RS22235 (L7H22_22235)	7879..8460	+	582	WP_029396071.1	hypothetical protein	-
L7H22_RS22240 (L7H22_22240)	8472..8981	+	510	WP_039022248.1	hypothetical protein	-
L7H22_RS22245 (L7H22_22245)	9257..10369	-	1113	WP_001300563.1	IS4-like element IS421 family transposase	-
L7H22_RS22250 (L7H22_22250)	10447..10602	-	156	WP_001352007.1	type I toxin-antitoxin system toxin HokD	-
L7H22_RS22255 (L7H22_22255)	10907..11683	-	777	Protein_10	RNA-guided endonuclease TnpB family protein	-
L7H22_RS22265 (L7H22_22265)	12429..12677	-	249	WP_223232864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L7H22_RS22270 (L7H22_22270)	12722..12991	-	270	WP_023338085.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
L7H22_RS22275 (L7H22_22275)	13955..14659	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
L7H22_RS22280 (L7H22_22280)	14665..15216	+	552	WP_097295927.1	hypothetical protein	-
L7H22_RS22285 (L7H22_22285)	15222..15899	+	678	WP_238094659.1	DsbA family protein	-
L7H22_RS22290 (L7H22_22290)	15951..16046	-	96	WP_233341282.1	DUF4113 domain-containing protein	-
L7H22_RS22295 (L7H22_22295)	16199..17158	-	960	Protein_18	Y-family DNA polymerase	-
L7H22_RS22300 (L7H22_22300)	17193..17561	-	369	WP_265414756.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A)	galE	1..90138	90138
	-	inside	IScluster/Tn	tet(A)	-	9094..30179	21085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (1-76)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9475.90 Da
• Isoelectric Point: 7.3132

>T234876 WP_223232864.1 NZ_CP091840:c12677-12429 [Escherichia coli]
IREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGARELVIHPHFVLVYEVDSQWGKVYILRVLHTTQKWVMLP
TY

Nucleotide

• Length: 249 bp

>T234876 NZ_LR134096:73632-73973 [Enterococcus faecium]
ATGACTGAAGAAAAAAATTACATTCCTAAAAAGGGAGATATTGTTTGGATTGATTTTGATCCTTCTGCAGGAAAAGAGAT
TCAAAAGCGGCGTCCTGGATTAGTTGTTTCTAGATACGAGTTTAATCGCAAAACAATGTTTGCAGTTATTTGTCCGATTA
CCTCTACTATAAGAAATTTTCCGACACGCTATTCATTACCTACCGAATTAGACACTACTGGACAAATACTTATTTCTCAA
TTGAAGTCGCTCGATTTCAATGAGAGAAAACTTAAAAAAATTGAATCCTTACCTTTACAAGATATGGCGAAAATCGATCA
AATGATTGAATATATATTTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10227.62 Da
• Isoelectric Point: 10.5807

>AT234876 WP_023338085.1 NZ_CP091840:c12991-12722 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQNNTWFRNQVAAGRQQLKRGDVLPHDMVESSAAAWRD
EMSRKVADK

Nucleotide

• Length: 270 bp

>AT234876 NZ_LR134096:73402-73632 [Enterococcus faecium]
ATGCTTATTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGATTACTTTGCCTTCTGATAACGGAAAGAAGCCTTCGGA
AAATAAAGAATATATAGTTATCTATTCTGATGATGGAACAATTACTTTGGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAAGCGGAGTATTACGAAAAAGATGAATGGGAAGATTTGTCTCCTGAAGGAAGAGAGATTTTATAA