Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4126104..4126722 Replicon chromosome
Accession NZ_CP091839
Organism Escherichia coli strain SWYHX138.261ft

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L7H22_RS19820 Protein ID WP_001291435.1
Coordinates 4126104..4126322 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L7H22_RS19825 Protein ID WP_000344800.1
Coordinates 4126348..4126722 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H22_RS19790 (4121995) 4121995..4122306 - 312 WP_050936577.1 MGMT family protein -
L7H22_RS19800 (4122685) 4122685..4123038 + 354 WP_238059090.1 DUF1428 family protein -
L7H22_RS19805 (4123080) 4123080..4124630 - 1551 WP_238059091.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L7H22_RS19810 (4124794) 4124794..4125264 - 471 WP_000136192.1 YlaC family protein -
L7H22_RS19815 (4125381) 4125381..4125932 - 552 WP_000102564.1 maltose O-acetyltransferase -
L7H22_RS19820 (4126104) 4126104..4126322 - 219 WP_001291435.1 HHA domain-containing protein Toxin
L7H22_RS19825 (4126348) 4126348..4126722 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L7H22_RS19830 (4127268) 4127268..4130417 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L7H22_RS19835 (4130440) 4130440..4131633 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4120637..4121965 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T234875 WP_001291435.1 NZ_CP091839:c4126322-4126104 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T234875 NZ_LR134095:513351-513692 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT234875 WP_000344800.1 NZ_CP091839:c4126722-4126348 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT234875 NZ_LR134095:513121-513351 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References