Detailed information of TA system TA023487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 11369..11960
• Replicon: plasmid pMNOD01
• Accession: NC_011892
• Organism: Methylobacterium nodulans ORS 2060

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B8IVI0
• Locus tag: MNOD_RS36400
• Protein ID: WP_015933972.1
• Coordinates: 11369..11674 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: MNOD_RS36405
• Protein ID: WP_015933973.1
• Coordinates: 11667..11960 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNOD_RS36365	6627..7031	-	405	WP_015933967.1	hypothetical protein	-
MNOD_RS36370	7375..7815	+	441	WP_015933968.1	DUF3291 domain-containing protein	-
MNOD_RS44655	7850..8020	-	171	WP_085985010.1	helix-turn-helix domain-containing protein	-
MNOD_RS36375	8488..8964	-	477	WP_015933969.1	VOC family protein	-
MNOD_RS36380	9325..9681	+	357	Protein_10	recombinase family protein	-
MNOD_RS48490	9630..9797	-	168	WP_198157692.1	hypothetical protein	-
MNOD_RS44665	10155..10871	-	717	WP_015933970.1	hypothetical protein	-
MNOD_RS36395	11023..11226	-	204	Protein_13	IS6 family transposase	-
MNOD_RS36400	11369..11674	+	306	WP_015933972.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MNOD_RS36405	11667..11960	+	294	WP_015933973.1	putative addiction module antidote protein	Antitoxin
MNOD_RS36410	12244..13524	+	1281	WP_012631399.1	IS66-like element ISMno2 family transposase	-
MNOD_RS36415	13656..14240	+	585	WP_015933974.1	NAD(P)H-binding protein	-
MNOD_RS36420	14413..15165	-	753	Protein_18	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..487734	487734

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (4-91)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11210.92 Da
• Isoelectric Point: 10.1761

>T23487 WP_015933972.1 NC_011892:11369-11674 [Methylobacterium nodulans ORS 2060]
VIELKQTATFAKWESRLRDKRARTIIAARLMRLAEGLPGDVEPVGEGVSELRIHYGPGYRVYFQQRGNLLIVLLCGGDKG
SQARDIAAAKKLAKEWSSQDA

Nucleotide

• Length: 306 bp

>T23487 NC_011892:11369-11674 [Methylobacterium nodulans ORS 2060]
GTGATTGAGTTAAAGCAGACGGCCACCTTCGCCAAATGGGAATCCCGCCTCCGGGACAAGCGAGCCAGAACCATTATCGC
GGCGCGATTGATGCGGCTGGCCGAAGGCTTGCCGGGCGACGTGGAGCCGGTAGGCGAAGGCGTTAGCGAACTGCGGATTC
ACTACGGCCCCGGATATCGCGTCTACTTCCAGCAGCGCGGAAATCTCCTGATCGTGTTGCTATGCGGCGGGGACAAGGGA
TCGCAAGCCCGCGACATTGCCGCCGCCAAGAAACTCGCCAAAGAATGGAGTTCACAAGATGCCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10363.83 Da
• Isoelectric Point: 5.8373

>AT23487 WP_015933973.1 NC_011892:11667-11960 [Methylobacterium nodulans ORS 2060]
MPEKLTTYDPAAALVDDEEIAAFMADAFETGDAAYVAKALGVVARAKGMTEISRKTGLSREQLYRSFSEHGNPTLKTTLA
VMRALGVDITAKARAAQ

Nucleotide

• Length: 294 bp

>AT23487 NC_011892:11667-11960 [Methylobacterium nodulans ORS 2060]
ATGCCTGAAAAACTGACCACTTACGACCCTGCCGCCGCACTGGTGGACGATGAGGAGATCGCCGCTTTCATGGCCGATGC
CTTTGAAACTGGCGATGCGGCCTATGTCGCCAAGGCGCTTGGCGTCGTCGCCCGCGCCAAAGGCATGACCGAAATATCCC
GCAAAACCGGGCTATCCCGTGAACAGCTTTACCGTTCGTTCAGCGAGCACGGAAACCCGACCCTCAAGACCACGCTTGCG
GTGATGCGCGCCCTGGGCGTTGACATAACGGCCAAAGCCCGTGCAGCACAGTGA

Structures

Toxin

• AlphaFold DB: B8IVI0