Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2101244..2101898 Replicon chromosome
Accession NZ_CP091839
Organism Escherichia coli strain SWYHX138.261ft

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag L7H22_RS10070 Protein ID WP_000244777.1
Coordinates 2101244..2101651 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L7H22_RS10075 Protein ID WP_000354046.1
Coordinates 2101632..2101898 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H22_RS10050 (2097201) 2097201..2098934 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
L7H22_RS10055 (2098940) 2098940..2099650 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L7H22_RS10060 (2099675) 2099675..2100571 - 897 WP_238059210.1 site-specific tyrosine recombinase XerD -
L7H22_RS10065 (2100683) 2100683..2101204 + 522 WP_001055874.1 flavodoxin FldB -
L7H22_RS10070 (2101244) 2101244..2101651 - 408 WP_000244777.1 protein YgfX Toxin
L7H22_RS10075 (2101632) 2101632..2101898 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L7H22_RS10080 (2102141) 2102141..2103121 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L7H22_RS10085 (2103317) 2103317..2103976 - 660 WP_000250274.1 hemolysin III family protein -
L7H22_RS10090 (2104140) 2104140..2104451 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
L7H22_RS10095 (2104496) 2104496..2105929 + 1434 WP_021571431.1 6-phospho-beta-glucosidase BglA -
L7H22_RS10100 (2105986) 2105986..2106729 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T234867 WP_000244777.1 NZ_CP091839:c2101651-2101244 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234867 NZ_LR134093:c2047490-2047314 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234867 WP_000354046.1 NZ_CP091839:c2101898-2101632 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234867 NZ_LR134093:2047191-2047246 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References