Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 52204..52805 Replicon plasmid pHX138.33gt_1
Accession NZ_CP091837
Organism Escherichia coli strain SWYHX138.33gt

Toxin (Protein)


Gene name doc Uniprot ID A0A5M4JUN5
Locus tag L7H17_RS22445 Protein ID WP_001216041.1
Coordinates 52204..52584 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag L7H17_RS22450 Protein ID WP_001190712.1
Coordinates 52584..52805 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H17_RS22415 (L7H17_22415) 48444..49472 + 1029 WP_166428522.1 tyrosine-type recombinase/integrase -
L7H17_RS22420 (L7H17_22420) 49545..49889 + 345 WP_004010537.1 hypothetical protein -
L7H17_RS22425 (L7H17_22425) 49886..50377 + 492 WP_134800774.1 hypothetical protein -
L7H17_RS22430 (L7H17_22430) 50370..50864 + 495 WP_110021427.1 dUTP diphosphatase -
L7H17_RS22435 (L7H17_22435) 50879..51319 + 441 WP_238059240.1 DUF2829 domain-containing protein -
L7H17_RS22440 (L7H17_22440) 51326..52120 + 795 WP_238059241.1 hypothetical protein -
L7H17_RS22445 (L7H17_22445) 52204..52584 - 381 WP_001216041.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
L7H17_RS22450 (L7H17_22450) 52584..52805 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
L7H17_RS22455 (L7H17_22455) 52878..53267 - 390 WP_238059242.1 S24 family peptidase -
L7H17_RS22460 (L7H17_22460) 53366..53617 - 252 WP_072644892.1 DNA polymerase III subunit theta -
L7H17_RS22465 (L7H17_22465) 53932..54336 - 405 WP_238059243.1 hypothetical protein -
L7H17_RS22470 (L7H17_22470) 54381..55376 - 996 WP_166428997.1 DUF968 domain-containing protein -
L7H17_RS22475 (L7H17_22475) 55522..56193 - 672 WP_238059244.1 metallophosphoesterase -
L7H17_RS22480 (L7H17_22480) 56190..56483 - 294 WP_064198790.1 DUF4406 domain-containing protein -
L7H17_RS22485 (L7H17_22485) 56483..56635 - 153 WP_166428979.1 hypothetical protein -
L7H17_RS22490 (L7H17_22490) 56756..57361 + 606 WP_166428996.1 Ref family recombination enhancement nuclease -
L7H17_RS22495 (L7H17_22495) 57603..57788 + 186 WP_134805653.1 host cell division inhibitor Icd-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..76063 76063


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13635.52 Da        Isoelectric Point: 5.6406

>T234854 WP_001216041.1 NZ_CP091837:c52584-52204 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELFGSNI

Download         Length: 381 bp

>T234854 NZ_LR134092:c3314124-3313750 [Escherichia coli]
ATGAAATTATTACCCGACACACATGTACGGGAGGCATCATGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGTCTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT234854 WP_001190712.1 NZ_CP091837:c52805-52584 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT234854 NZ_LR134092:c3314568-3314215 [Escherichia coli]
GTGTCAGACACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGACGCCCTGTTTCGGCGC
CCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGTTCAGCGATGCAGATG
CGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGTGAACTGAATCCCCGC
CATCAGCATACCGTCACGCTATATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCT
GGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5M4JUN5

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References