Detailed information of TA system TA023485

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 128880..129478
• Replicon: plasmid pMNOD02
• Accession: NC_011887
• Organism: Methylobacterium nodulans ORS 2060

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B8IWM2
• Locus tag: MNOD_RS39020
• Protein ID: WP_012631116.1
• Coordinates: 129185..129478 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B8IVS9
• Locus tag: MNOD_RS39015
• Protein ID: WP_012631115.1
• Coordinates: 128880..129188 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MNOD_RS39005	124727..125896	+	1170	WP_012631113.1	CoA transferase	-
MNOD_RS45155	126370..126585	+	216	WP_083786741.1	helix-turn-helix transcriptional regulator	-
MNOD_RS45160	126965..127384	+	420	WP_198157707.1	MaoC family dehydratase	-
MNOD_RS45165	127536..128613	-	1078	Protein_116	IS1380 family transposase	-
MNOD_RS39015	128880..129188	+	309	WP_012631115.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MNOD_RS39020	129185..129478	+	294	WP_012631116.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
MNOD_RS45175	129705..129995	-	291	Protein_119	alanine racemase	-
MNOD_RS45180	130044..130210	-	167	Protein_120	ornithine cyclodeaminase family protein	-
MNOD_RS39025	130245..130379	-	135	Protein_121	helix-turn-helix domain-containing protein	-
MNOD_RS39030	130527..131762	-	1236	WP_012631117.1	DUF1612 domain-containing protein	-
MNOD_RS39035	131937..132998	+	1062	WP_012631118.1	tyrosine-type recombinase/integrase	-
MNOD_RS45185	133595..134050	+	456	WP_012631119.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..458070	458070

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-55)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10594.04 Da
• Isoelectric Point: 6.4640

>T23485 WP_012631116.1 NC_011887:129185-129478 [Methylobacterium nodulans ORS 2060]
VKLVWSAFALSDRGGIFTHIEADNPAAAIVIDERIVAATRRLRDFPESGRPGRIAGTRELVVTGTPYIAAYQVTGETVRI
LRVLHGAQQWPDDLPEG

Nucleotide

• Length: 294 bp

>T23485 NC_011887:129185-129478 [Methylobacterium nodulans ORS 2060]
GTGAAGCTCGTCTGGTCGGCCTTCGCGCTTTCGGATCGCGGCGGCATCTTCACGCATATCGAGGCCGACAACCCTGCGGC
CGCCATTGTCATCGACGAGCGGATCGTGGCCGCGACCCGCCGTCTTCGGGACTTTCCCGAAAGTGGCCGACCCGGCCGCA
TTGCCGGAACCCGCGAGCTTGTCGTTACCGGGACGCCCTACATCGCGGCCTATCAAGTGACCGGAGAGACAGTCCGAATC
CTCCGTGTGCTGCACGGGGCGCAACAGTGGCCGGACGATCTCCCCGAGGGCTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11190.55 Da
• Isoelectric Point: 5.1663

>AT23485 WP_012631115.1 NC_011887:128880-129188 [Methylobacterium nodulans ORS 2060]
MPANALVQTRIDADVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELVTNSEAHDAWFRAKVLEALDDTRPDVPDD
QAEAHFAQRRAAARRRAGELKS

Nucleotide

• Length: 309 bp

>AT23485 NC_011887:128880-129188 [Methylobacterium nodulans ORS 2060]
ATGCCTGCAAACGCACTCGTTCAGACACGCATCGACGCTGACGTCCGGGATCGCGCATCGGCGGTGCTCGAGAACATGGG
CCTTACGGTCTCGGATGCGGTGCGCATTCTGCTCACCCGGACCGCCAATGAGGGTGCGCTCCCCCTCGAGCTGGTCACGA
ACAGCGAGGCGCACGACGCCTGGTTCCGGGCCAAGGTCCTTGAGGCGCTGGACGACACCCGGCCCGACGTTCCTGACGAT
CAGGCTGAGGCGCATTTCGCGCAGCGGCGCGCTGCGGCCCGGCGCAGGGCCGGCGAACTGAAATCGTGA

Structures

Toxin

• AlphaFold DB: B8IWM2

Antitoxin

• AlphaFold DB: B8IVS9