Detailed information of TA system TA234849

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 3453361..3453858
• Replicon: chromosome
• Accession: NZ_CP091836
• Organism: Escherichia coli strain SWYHX138.33gt

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: L7H17_RS16590
• Protein ID: WP_001173343.1
• Coordinates: 3453685..3453858 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: L7H17_RS16585
• Protein ID: WP_095716590.1
• Coordinates: 3453361..3453657 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7H17_RS16565 (3450092)	3450092..3450811	-	720	WP_000611288.1	two-component system response regulator DcuR	-
L7H17_RS16570 (3450808)	3450808..3452439	-	1632	WP_001216477.1	sensor histidine kinase	-
L7H17_RS16575 (3452620)	3452620..3452850	+	231	WP_000371704.1	4Fe-4S mono-cluster protein YjdI	-
L7H17_RS16580 (3452862)	3452862..3453134	+	273	WP_000405647.1	GNAT family N-acetyltransferase	-
L7H17_RS16585 (3453361)	3453361..3453657	+	297	WP_095716590.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
L7H17_RS16590 (3453685)	3453685..3453858	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
L7H17_RS16595 (3453977)	3453977..3455494	-	1518	WP_112911841.1	lysine--tRNA ligase	-
L7H17_RS16600 (3455731)	3455731..3457188	-	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T234849 WP_001173343.1 NZ_CP091836:3453685-3453858 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T234849 NZ_LR134092:2564704-2566026 [Escherichia coli]
ATGCCCAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACTTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCTCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGGGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGGAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGAATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGGTATG
ATTTTATGAAATTCCAGGTCTTTCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGTACTGGATAACGTGAAGACTTCATTACCAACAGATTTTCCAGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11495.04 Da
• Isoelectric Point: 4.2931

>AT234849 WP_095716590.1 NZ_CP091836:3453361-3453657 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMKVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT234849 NZ_LR134092:2564438-2564704 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAAAAGCAAGATCTGGAGTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41