Detailed information of TA system TA023484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 1997596..1999283
• Replicon: chromosome
• Accession: NC_011852
• Organism: Haemophilus parasuis SH0165

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B8F842
• Locus tag: HAPS_RS09865
• Protein ID: WP_015940034.1
• Coordinates: 1997596..1998939 (+)
• Length: 448 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HAPS_RS09870
• Protein ID: WP_015940035.1
• Coordinates: 1998939..1999283 (+)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HAPS_RS09840	1992599..1994776	-	2178	WP_010787252.1	bifunctional 23S rRNA (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA (guanine(2445)-N(2))-methyltransferase RlmL	-
HAPS_RS09845	1994926..1995867	-	942	WP_015940030.1	16S rRNA (guanine(1207)-N(2))-methyltransferase RsmC	-
HAPS_RS09850	1995950..1996363	+	414	WP_015940031.1	DNA polymerase III subunit psi	-
HAPS_RS09855	1996365..1996805	+	441	WP_015940032.1	M23 family metallopeptidase	-
HAPS_RS09860	1997047..1997484	+	438	WP_015940033.1	ribosomal protein S18-alanine N-acetyltransferase	-
HAPS_RS09865	1997596..1998939	+	1344	WP_015940034.1	type II toxin-antitoxin system HipA family toxin	Toxin
HAPS_RS09870	1998939..1999283	+	345	WP_015940035.1	helix-turn-helix domain-containing protein	Antitoxin
HAPS_RS09875	1999312..2000637	+	1326	WP_015940036.1	ATP-dependent RNA helicase SrmB	-
HAPS_RS09880	2000758..2001390	+	633	WP_015940037.1	lipoprotein insertase outer membrane protein LolB	-
HAPS_RS09885	2001392..2002237	+	846	WP_015940038.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
HAPS_RS09890	2002265..2003215	+	951	WP_005713153.1	ribose-phosphate pyrophosphokinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (161-407)

Antitoxin

• Cro/C1-type helix-turn-helix domain (43-92)

Sequences

Toxin

Protein

• Length: 448 a.a.
• Molecular weight: 51037.05 Da
• Isoelectric Point: 5.1119

>T23484 WP_015940034.1 NC_011852:1997596-1998939 [Glaesserella parasuis SH0165]
MQIEPLTLQAYIADRWQDIAILTFQADYSLNDFSYLYDLTEILSDNDKAVSFNYPVEFFPHRSVSGWFAFLDDIVPSGAS
RRYWLQKLDIAHLPMAQQNYLLLKQGTIAPVGHLRVKQAVSSFIENEPPRLFEIDDVINRQADFLEYANQRGAMAGGATG
AGGEAPKLLLRQTATQQVWIDNLQQGDAADDFYLVKFPRGKRSAIDCDILRAEFHYYHELASMGFDTIDTTKMRLEEGER
YPSLWLPRFDVYRNEQGKMARYAVESVYAMLEKGAGSLLDHEKVIRQLIAKIENSHLVETGMAFDKQAFVIEWVRRDLLN
IAFGNSDNHGRNTAFLRNQNRIWLAPIYDFAPMKADPEGIPRTTMWDSKGHPALELGGEYRFDLIAEQLDDLCPADKLLD
ALRQTAQQLVGLKARLEARGVPTAILTYPSISLDYLPEKLKRWQLLS

Nucleotide

• Length: 1344 bp

>T23484 NC_011852:1997596-1998939 [Glaesserella parasuis SH0165]
ATGCAAATCGAACCGCTTACACTTCAGGCCTATATTGCTGATCGTTGGCAGGATATTGCTATTTTGACCTTTCAGGCTGA
TTATAGTCTCAATGATTTTAGCTATCTCTATGATTTAACAGAAATTTTAAGTGATAATGATAAGGCAGTTTCATTTAACT
ACCCTGTGGAATTTTTTCCGCATCGCAGTGTATCGGGCTGGTTTGCATTTTTAGATGATATAGTCCCTTCTGGGGCAAGC
CGGCGTTATTGGTTGCAAAAACTAGATATTGCTCATTTGCCGATGGCTCAACAAAACTATTTGTTATTAAAACAAGGCAC
GATTGCACCTGTCGGGCATTTACGAGTTAAACAAGCGGTCAGTTCTTTTATCGAAAATGAACCCCCTCGCTTATTTGAAA
TAGATGATGTTATCAATCGCCAAGCTGATTTTTTGGAATATGCTAATCAGCGTGGGGCAATGGCTGGCGGTGCAACGGGG
GCTGGTGGCGAAGCGCCGAAATTATTGCTACGCCAAACAGCCACTCAGCAAGTTTGGATTGATAATCTACAACAAGGTGA
CGCTGCCGATGATTTCTATTTAGTCAAGTTTCCGCGTGGAAAACGCAGTGCAATAGATTGCGACATATTGAGAGCTGAAT
TTCACTATTACCACGAATTAGCCTCAATGGGTTTTGATACCATTGATACGACAAAAATGCGGTTGGAAGAGGGCGAACGT
TACCCGTCGTTATGGTTACCTCGCTTTGATGTTTATCGTAATGAACAGGGGAAAATGGCTCGTTATGCCGTTGAATCTGT
TTATGCTATGTTAGAAAAAGGGGCTGGCTCTTTGCTGGATCACGAAAAGGTTATTCGTCAGTTGATTGCCAAAATTGAAA
ATTCTCACCTAGTTGAAACAGGAATGGCTTTTGATAAACAAGCTTTTGTGATCGAGTGGGTAAGGCGAGATTTATTGAAT
ATCGCCTTTGGAAATAGCGATAATCACGGGCGAAATACGGCTTTCTTACGCAATCAAAATCGTATTTGGCTTGCTCCAAT
TTATGATTTTGCCCCAATGAAAGCAGATCCTGAAGGTATTCCTCGCACGACAATGTGGGATAGTAAAGGTCACCCTGCAT
TAGAATTAGGCGGTGAATATCGTTTTGATCTGATTGCAGAACAGCTTGATGATTTATGTCCAGCCGACAAGTTATTGGAT
GCTTTGCGTCAAACGGCTCAACAGCTTGTCGGACTTAAAGCACGTTTAGAAGCAAGGGGCGTTCCAACGGCGATTTTGAC
GTATCCTTCAATCAGTTTGGACTATTTACCTGAAAAATTAAAACGTTGGCAATTATTATCATAA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 13225.52 Da
• Isoelectric Point: 10.2782

>AT23484 WP_015940035.1 NC_011852:1998939-1999283 [Glaesserella parasuis SH0165]
MEEIKQARASKSKKTLDIYQRATVRDEIARKLLLNEMSLGQALKYLRLHLLAMKQERYAEIVKVSRKTLSDLENDKGNYS
IDIINQVLRPFELQLGVVPMNKTLLRQVLNEQAV

Nucleotide

• Length: 345 bp

>AT23484 NC_011852:1998939-1999283 [Glaesserella parasuis SH0165]
ATGGAAGAGATCAAACAGGCGAGAGCCTCTAAAAGTAAAAAAACGTTAGATATTTATCAGAGAGCGACTGTGCGAGACGA
AATTGCTCGTAAGCTATTGCTCAATGAGATGAGTTTAGGGCAGGCGTTGAAATATCTACGTTTACATCTACTGGCAATGA
AACAAGAGCGTTATGCGGAGATTGTCAAAGTTTCTCGCAAAACGTTATCTGATTTAGAAAATGATAAGGGAAATTACAGT
ATTGATATTATCAATCAGGTGTTACGCCCCTTTGAATTACAACTAGGTGTTGTGCCAATGAACAAAACACTACTCAGACA
AGTTTTAAACGAACAAGCGGTCTGA

Structures

Toxin

• AlphaFold DB: B8F842