Detailed information of TA system TA234832

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): sanaTA/AbiEii-DUF6088
• Location: 221602..222950
• Replicon: plasmid unnamed
• Accession: NZ_CP091834
• Organism: Shewanella xiamenensis strain HD6452

Toxin (Protein)

• Gene name: sanaT
• Uniprot ID: -
• Locus tag: K5M76_RS22775
• Protein ID: WP_259476961.1
• Coordinates: 222009..222950 (+)
• Length: 314 a.a.

Antitoxin (Protein)

• Gene name: sanaA
• Uniprot ID: A0A5B8R5A0
• Locus tag: K5M76_RS22770
• Protein ID: WP_011711497.1
• Coordinates: 221602..222012 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5M76_RS22750 (K5M76_22715)	217004..217183	-	180	WP_014611668.1	ribosome modulation factor	-
K5M76_RS22755 (K5M76_22720)	217502..219094	-	1593	WP_014611666.1	conjugal transfer protein TraG N-terminal domain-containing protein	-
K5M76_RS22760 (K5M76_22725)	219094..220443	-	1350	WP_195742685.1	hypothetical protein	-
K5M76_RS22765 (K5M76_22730)	220693..221037	-	345	Protein_242	DUF4113 domain-containing protein	-
K5M76_RS22770 (K5M76_22735)	221602..222012	+	411	WP_011711497.1	DUF6088 family protein	Antitoxin
K5M76_RS22775 (K5M76_22740)	222009..222950	+	942	WP_259476961.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
K5M76_RS22780 (K5M76_22745)	222958..225600	+	2643	WP_208660715.1	type I restriction-modification system subunit M	-
K5M76_RS22785 (K5M76_22750)	225602..226882	+	1281	WP_218679072.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ARR-3 / dfrA27 / aadA16 / qacE / sul1 / blaNDM-1 / qnrVC6 / blaCMY-10 / mph(A) / blaTEM-1B / aac(3)-IId / msr(E) / mph(E) / tet(C) / aadA6	-	1..256918	256918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (37-299)

Antitoxin

• Protein of unknown function DUF6088 (8-130)

Sequences

Toxin

Protein

• Length: 314 a.a.
• Molecular weight: 35737.62 Da
• Isoelectric Point: 7.0965

>T234832 WP_259476961.1 NZ_CP091834:222009-222950 [Shewanella xiamenensis]
MKKLKQQFLEVSDALALGNPAIIEKDYWVVALLSELEQLTFETHQMVFSGGTALAKSNVKILRMSEDVDIKLIPTPQFQH
LSRGRKKQYRKALAVKIETAIIASNTFSIEARLVQDEYRYVEYELKYPQQLSQAPCLRPIIKLELMETIPLLALETRGIQ
SLVAELYKLPQEVNGFICVSVNTTLVEKVLSMLRRTMSVKRDAARSDDVTLVRHIYDVHCIHAAQALDNELLAPLFRAIL
AEDIARFGNQHAEFVMDPANELRLGLQEIESNPLFRQRFNDFVKPMVFNSQPPNFDTCFMSFKHIATSLILTI

Nucleotide

• Length: 942 bp

>T234832 NZ_LR134091:c2409710-2409444 [Staphylococcus aureus]
ATGGCTAGGTTAAATATTACGTTTTCGCCTCAAGCCTTTGAAGATTATAAGTATTTTCAGCAGAACGATAAAAAAATGGT
GAAGAAGATTAATGAGTTACTTAAAAGTATTGACAGAAATGGTGCATTGGAAGGTATAGGTAAGCCTGAAAAGTTAAAAT
CGAATCTGACTGGGTATTATAGTAGACGTATCAATCACGAACATAGATTGGTTTATACAGTAGATGACAATCATATAAAA
ATAGCATCATGTAAATACCATTATTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15491.86 Da
• Isoelectric Point: 10.3652

>AT234832 WP_011711497.1 NZ_CP091834:221602-222012 [Shewanella xiamenensis]
MAAVDSIFQKIKRSRRYVFERKDFDGIASYDQIGRSLNQLMDRGEIMKLGYGLYTKARVNSLTGRLMPSCPGGSDALMRE
ILKMKDIDFEIDNLSLQSLSGKNTQIPSSVHYSWDQKQFNRKLVVGNRLLTPASRP

Nucleotide

• Length: 411 bp

>AT234832 NZ_LR134091:c2409961-2409710 [Staphylococcus aureus]
ATGATTATTAAAAATTATTCATACGCTCGACAGAATTTAAAGGCACTTATGACAAAAGTAAATGATGATAGTGATATGGT
AACTGTAACATCTACTGATGATAAAAACGTAGTAATCATGTCAGAATCAGATTATAACTCCATGATGGAAACACTTTACC
TCCAACAGAACCCAAATAATGCTGAACACTTAGCTCAATCAATTGCAGATCTAGAACGTGGGAAAACTATAACGAAAGAT
ATAGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A5B8R5A0