Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 291642..292312 Replicon plasmid pFQ61_ST383_NDM-5
Accession NZ_CP091814
Organism Klebsiella pneumoniae strain 10MC160002271

Toxin (Protein)


Gene name vagD Uniprot ID Q6U619
Locus tag L7F76_RS27775 Protein ID WP_004213072.1
Coordinates 291642..292085 (-) Length 148 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q6U620
Locus tag L7F76_RS27780 Protein ID WP_004213073.1
Coordinates 292082..292312 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7F76_RS27740 (L7F76_27685) 287049..287324 + 276 WP_004213932.1 carboxymuconolactone decarboxylase family protein -
L7F76_RS27745 (L7F76_27690) 287387..287878 + 492 WP_011251327.1 DM13 domain-containing protein -
L7F76_RS27750 (L7F76_27695) 287927..288847 + 921 WP_004213934.1 DUF1471 domain-containing protein -
L7F76_RS27755 (L7F76_27700) 288938..289341 + 404 Protein_309 GAF domain-containing protein -
L7F76_RS27760 (L7F76_27705) 289860..290498 - 639 Protein_310 mucoid phenotype regulator RmpA2 -
L7F76_RS27765 (L7F76_27710) 290915..291219 + 305 Protein_311 transposase -
L7F76_RS27770 (L7F76_27715) 291242..291493 - 252 WP_186987481.1 hypothetical protein -
L7F76_RS27775 (L7F76_27720) 291642..292085 - 444 WP_004213072.1 type II toxin-antitoxin system VapC family toxin Toxin
L7F76_RS27780 (L7F76_27725) 292082..292312 - 231 WP_004213073.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L7F76_RS27785 (L7F76_27730) 292920..294053 + 1134 WP_004213075.1 DUF3800 domain-containing protein -
L7F76_RS27790 (L7F76_27735) 294069..294362 + 294 WP_004213076.1 hypothetical protein -
L7F76_RS27795 (L7F76_27740) 294352..294558 - 207 WP_004213077.1 hypothetical protein -
L7F76_RS27800 (L7F76_27745) 294910..295200 + 291 WP_004213078.1 hypothetical protein -
L7F76_RS27805 (L7F76_27750) 295190..296089 + 900 WP_004225022.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-5 / aph(3')-VI / qnrS1 / blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1B / aph(3')-Ia / sul2 / armA / msr(E) / mph(E) / dfrA5 / qacE / sul1 / mph(A) rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..376754 376754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16006.74 Da        Isoelectric Point: 9.3642

>T234824 WP_004213072.1 NZ_CP091814:c292085-291642 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Download         Length: 444 bp

>T234824 NZ_LR134090:c2245439-2245335 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8677.73 Da        Isoelectric Point: 4.4563

>AT234824 WP_004213073.1 NZ_CP091814:c292312-292082 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT234824 NZ_LR134090:2245241-2245279 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q6U619

Antitoxin

Source ID Structure
AlphaFold DB Q6U620

References