Detailed information of TA system TA234823

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 139962..140872
• Replicon: plasmid pFQ61_ST383_NDM-5
• Accession: NZ_CP091814
• Organism: Klebsiella pneumoniae strain 10MC160002271

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A663AYG0
• Locus tag: L7F76_RS26950
• Protein ID: WP_004026354.1
• Coordinates: 140402..140872 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: L7F76_RS26945
• Protein ID: WP_004026357.1
• Coordinates: 139962..140405 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7F76_RS26930 (L7F76_26890)	136596..138083	+	1488	WP_020803845.1	group II intron reverse transcriptase/maturase	-
L7F76_RS26935 (L7F76_26895)	138169..138591	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
L7F76_RS26940 (L7F76_26900)	138591..139862	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
L7F76_RS26945 (L7F76_26905)	139962..140405	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
L7F76_RS26950 (L7F76_26910)	140402..140872	+	471	WP_004026354.1	RES family NAD+ phosphorylase	Toxin
L7F76_RS26955 (L7F76_26915)	140981..141241	-	261	WP_004026352.1	hypothetical protein	-
L7F76_RS26960 (L7F76_26920)	141964..142122	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
L7F76_RS26970 (L7F76_26930)	143434..143886	+	453	Protein_152	CatA-like O-acetyltransferase	-
L7F76_RS26975 (L7F76_26935)	144087..144464	-	378	WP_000656305.1	GNAT family N-acetyltransferase	-
L7F76_RS26980 (L7F76_26940)	144775..145779	-	1005	WP_000427619.1	IS110-like element IS5075 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaNDM-5 / aph(3')-VI / qnrS1 / blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1B / aph(3')-Ia / sul2 / armA / msr(E) / mph(E) / dfrA5 / qacE / sul1 / mph(A)	rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..376754	376754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17515.79 Da
• Isoelectric Point: 4.6155

>T234823 WP_004026354.1 NZ_CP091814:140402-140872 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T234823 NZ_LR134090:c2188080-2187871 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACATCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT234823 WP_004026357.1 NZ_CP091814:139962-140405 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT234823 NZ_LR134090:c2188385-2188092 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CATTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A663AYG0

Antitoxin

• AlphaFold DB: A0A6P1V3Q9