234822

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-DnaT
Location 22939..23461 Replicon plasmid pFQ61_ST383_NDM-5
Accession NZ_CP091814
Organism Klebsiella pneumoniae strain 10MC160002271

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag L7F76_RS26340 Protein ID WP_004181778.1
Coordinates 23177..23461 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A5Q9LM53
Locus tag L7F76_RS26335 Protein ID WP_025368601.1
Coordinates 22939..23187 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7F76_RS26315 (L7F76_26280) 18148..18984 - 837 WP_223686625.1 hypothetical protein -
L7F76_RS26320 (L7F76_26285) 19031..19384 - 354 WP_004181774.1 hypothetical protein -
L7F76_RS26325 (L7F76_26290) 19529..20515 - 987 WP_025368599.1 hypothetical protein -
L7F76_RS26330 (L7F76_26295) 20849..22648 - 1800 WP_025368600.1 ATP-dependent helicase -
L7F76_RS26335 (L7F76_26300) 22939..23187 + 249 WP_025368601.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
L7F76_RS26340 (L7F76_26305) 23177..23461 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L7F76_RS26345 (L7F76_26310) 23691..24917 + 1227 WP_025368602.1 RNA-guided endonuclease TnpB family protein -
L7F76_RS26350 (L7F76_26315) 25203..25628 - 426 WP_040120489.1 IS200/IS605 family transposase -
L7F76_RS26355 (L7F76_26320) 25665..26891 + 1227 WP_025368604.1 RNA-guided endonuclease TnpB family protein -
L7F76_RS26360 (L7F76_26325) 26957..28083 - 1127 Protein_30 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-5 / aph(3')-VI / qnrS1 / blaCTX-M-15 / aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1B / aph(3')-Ia / sul2 / armA / msr(E) / mph(E) / dfrA5 / qacE / sul1 / mph(A) rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..376754 376754


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T234822 WP_004181778.1 NZ_CP091814:23177-23461 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T234822 NZ_LR134090:c2168486-2168124 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8906.12 Da        Isoelectric Point: 3.8206

>AT234822 WP_025368601.1 NZ_CP091814:22939-23187 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT234822 NZ_LR134090:c2168653-2168483 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LM53

References