Detailed information of TA system TA234821

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5236680..5237305
• Replicon: chromosome
• Accession: NZ_CP091813
• Organism: Klebsiella pneumoniae strain 10MC160002271

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L7F76_RS25845
• Protein ID: WP_062921072.1
• Coordinates: 5236680..5237063 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: L7F76_RS25850
• Protein ID: WP_004150355.1
• Coordinates: 5237063..5237305 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7F76_RS25830 (5234046)	5234046..5234948	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
L7F76_RS25835 (5234945)	5234945..5235580	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
L7F76_RS25840 (5235577)	5235577..5236506	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
L7F76_RS25845 (5236680)	5236680..5237063	-	384	WP_062921072.1	type II toxin-antitoxin system VapC family toxin	Toxin
L7F76_RS25850 (5237063)	5237063..5237305	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
L7F76_RS25855 (5237510)	5237510..5238427	+	918	WP_062921073.1	alpha/beta hydrolase	-
L7F76_RS25860 (5238441)	5238441..5239382	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
L7F76_RS25865 (5239427)	5239427..5239864	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
L7F76_RS25870 (5239861)	5239861..5240721	-	861	WP_004146232.1	virulence factor BrkB family protein	-
L7F76_RS25875 (5240715)	5240715..5241314	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14377.62 Da
• Isoelectric Point: 7.3178

>T234821 WP_062921072.1 NZ_CP091813:c5237063-5236680 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDALILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T234821 NZ_LR134090:c1949731-1949579 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT234821 WP_004150355.1 NZ_CP091813:c5237305-5237063 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT234821 NZ_LR134090:c1950355-1949756 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCTGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9