Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4633196..4634006 Replicon chromosome
Accession NZ_CP091813
Organism Klebsiella pneumoniae strain 10MC160002271

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag L7F76_RS23010 Protein ID WP_004178461.1
Coordinates 4633196..4633729 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag L7F76_RS23015 Protein ID WP_002887278.1
Coordinates 4633740..4634006 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7F76_RS23005 (4632027) 4632027..4633148 + 1122 WP_002887282.1 cupin domain-containing protein -
L7F76_RS23010 (4633196) 4633196..4633729 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
L7F76_RS23015 (4633740) 4633740..4634006 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
L7F76_RS23020 (4634109) 4634109..4635542 - 1434 WP_062921017.1 Cu(+)/Ag(+) sensor histidine kinase -
L7F76_RS23025 (4635532) 4635532..4636215 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
L7F76_RS23030 (4636387) 4636387..4637772 + 1386 WP_062921018.1 efflux transporter outer membrane subunit -
L7F76_RS23035 (4637790) 4637790..4638134 + 345 WP_025862174.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T234819 WP_004178461.1 NZ_CP091813:c4633729-4633196 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T234819 NZ_LR134090:c870522-870334 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAATCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTACTATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT234819 WP_002887278.1 NZ_CP091813:c4634006-4633740 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT234819 NZ_LR134090:c870811-870551 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATTGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References