Detailed information of TA system TA234802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 147782..148425
• Replicon: plasmid p1_21223
• Accession: NZ_CP091806
• Organism: Klebsiella quasipneumoniae strain 21223

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: L7H25_RS26265
• Protein ID: WP_268199256.1
• Coordinates: 147782..148198 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: L7H25_RS26270
• Protein ID: WP_001261276.1
• Coordinates: 148195..148425 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L7H25_RS26215 (L7H25_26210)	143753..144025	+	273	Protein_157	transposase	-
L7H25_RS26220 (L7H25_26215)	144324..144578	-	255	WP_032731922.1	hypothetical protein	-
L7H25_RS26225 (L7H25_26220)	144654..144911	-	258	WP_004098928.1	hypothetical protein	-
L7H25_RS26230 (L7H25_26225)	144960..145163	-	204	WP_004150739.1	HHA domain-containing protein	-
L7H25_RS26235 (L7H25_26230)	145224..145718	-	495	WP_016528789.1	hypothetical protein	-
L7H25_RS26240 (L7H25_26235)	145749..146315	-	567	WP_009654312.1	hypothetical protein	-
L7H25_RS26245 (L7H25_26240)	146312..146575	-	264	WP_004118691.1	hypothetical protein	-
L7H25_RS26250 (L7H25_26245)	146815..147141	-	327	WP_124989605.1	hypothetical protein	-
L7H25_RS26255 (L7H25_26250)	147220..147438	-	219	WP_021312527.1	hypothetical protein	-
L7H25_RS26260 (L7H25_26255)	147502..147747	-	246	WP_065905238.1	hypothetical protein	-
L7H25_RS26265 (L7H25_26260)	147782..148198	-	417	WP_268199256.1	type II toxin-antitoxin system VapC family toxin	Toxin
L7H25_RS26270 (L7H25_26265)	148195..148425	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
L7H25_RS26275 (L7H25_26270)	149030..149323	+	294	WP_124989603.1	hypothetical protein	-
L7H25_RS26280 (L7H25_26275)	149372..149845	+	474	WP_065905236.1	hypothetical protein	-
L7H25_RS26285 (L7H25_26280)	149847..150815	+	969	WP_078207718.1	IS5 family transposase	-
L7H25_RS26290 (L7H25_26285)	151502..152302	-	801	WP_065905270.1	EAL domain-containing protein	-
L7H25_RS26295 (L7H25_26290)	152402..153082	-	681	Protein_173	CSS-motif domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..164397	164397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15082.49 Da
• Isoelectric Point: 7.8644

>T234802 WP_268199256.1 NZ_CP091806:c148198-147782 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T234802 NZ_LR134086:c2182084-2181875 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT234802 WP_001261276.1 NZ_CP091806:c148425-148195 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT234802 NZ_LR134086:c2182389-2182096 [Staphylococcus aureus]
ATGACTGAACAAGATATTGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A387K023