Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 79679..80415 Replicon plasmid p1_21223
Accession NZ_CP091806
Organism Klebsiella quasipneumoniae strain 21223

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag L7H25_RS25885 Protein ID WP_003026803.1
Coordinates 79679..80161 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L7H25_RS25890 Protein ID WP_003026799.1
Coordinates 80149..80415 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L7H25_RS25865 (L7H25_25860) 74974..75678 - 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
L7H25_RS25870 (L7H25_25865) 75838..77184 - 1347 WP_077251107.1 ISNCY family transposase -
L7H25_RS25875 (L7H25_25870) 77414..78046 + 633 WP_001567369.1 hypothetical protein -
L7H25_RS25880 (L7H25_25875) 78075..79478 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
L7H25_RS25885 (L7H25_25880) 79679..80161 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
L7H25_RS25890 (L7H25_25885) 80149..80415 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L7H25_RS25895 (L7H25_25890) 80825..81793 - 969 WP_072202995.1 IS5 family transposase -
L7H25_RS25900 (L7H25_25895) 81853..82335 + 483 WP_004205976.1 helix-turn-helix domain-containing protein -
L7H25_RS25905 (L7H25_25900) 82371..83069 - 699 WP_004205977.1 urea ABC transporter ATP-binding subunit UrtE -
L7H25_RS25910 (L7H25_25905) 83079..83876 - 798 WP_004205978.1 urea ABC transporter ATP-binding protein UrtD -
L7H25_RS25915 (L7H25_25910) 83876..84949 - 1074 WP_017900354.1 urea ABC transporter permease subunit UrtC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..164397 164397
- inside IScluster/Tn - - 75838..81793 5955


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T234801 WP_003026803.1 NZ_CP091806:c80161-79679 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T234801 NZ_LR134086:c2162482-2162120 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATTATTCAAAATGACACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAATATAAATTGGATAAAGACTCAGTCATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTATTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT234801 WP_003026799.1 NZ_CP091806:c80415-80149 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT234801 NZ_LR134086:c2162649-2162479 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAATCATATTTATCTTCTAATTCAA
CGAATGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References