Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3493256..3493874 Replicon chromosome
Accession NZ_CP091773
Organism Escherichia coli strain SWYH.B349aT

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L6V99_RS17080 Protein ID WP_001291435.1
Coordinates 3493656..3493874 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L6V99_RS17075 Protein ID WP_000344800.1
Coordinates 3493256..3493630 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V99_RS17065 (3488345) 3488345..3489538 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6V99_RS17070 (3489561) 3489561..3492710 + 3150 WP_237877960.1 efflux RND transporter permease AcrB -
L6V99_RS17075 (3493256) 3493256..3493630 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L6V99_RS17080 (3493656) 3493656..3493874 + 219 WP_001291435.1 HHA domain-containing protein Toxin
L6V99_RS17085 (3494046) 3494046..3494597 + 552 WP_000102568.1 maltose O-acetyltransferase -
L6V99_RS17090 (3494713) 3494713..3495183 + 471 WP_000136192.1 YlaC family protein -
L6V99_RS17095 (3495347) 3495347..3496897 + 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L6V99_RS17100 (3496939) 3496939..3497292 - 354 WP_000878140.1 DUF1428 domain-containing protein -
L6V99_RS17110 (3497671) 3497671..3497982 + 312 WP_000409911.1 MGMT family protein -
L6V99_RS17115 (3498013) 3498013..3498585 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T234754 WP_001291435.1 NZ_CP091773:3493656-3493874 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T234754 NZ_LR134080:2351364-2351651 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT234754 WP_000344800.1 NZ_CP091773:3493256-3493630 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT234754 NZ_LR134080:2351125-2351364 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References