Detailed information of TA system TA234743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1355282..1355907
• Replicon: chromosome
• Accession: NZ_CP091773
• Organism: Escherichia coli strain SWYH.B349aT

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L6V99_RS06565
• Protein ID: WP_000911330.1
• Coordinates: 1355509..1355907 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: L6V99_RS06560
• Protein ID: WP_000450524.1
• Coordinates: 1355282..1355509 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6V99_RS06535 (1351085)	1351085..1351555	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
L6V99_RS06540 (1351555)	1351555..1352127	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
L6V99_RS06545 (1352273)	1352273..1353151	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
L6V99_RS06550 (1353168)	1353168..1354202	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
L6V99_RS06555 (1354415)	1354415..1355128	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
L6V99_RS06560 (1355282)	1355282..1355509	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
L6V99_RS06565 (1355509)	1355509..1355907	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
L6V99_RS06570 (1356054)	1356054..1356917	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
L6V99_RS06575 (1356932)	1356932..1358947	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
L6V99_RS06580 (1359021)	1359021..1359719	+	699	WP_000679823.1	esterase	-
L6V99_RS06585 (1359829)	1359829..1360029	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T234743 WP_000911330.1 NZ_CP091773:1355509-1355907 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T234743 NZ_LR134079:c4974051-4973866 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTCTCTCTTTTAGGAGCTGTAGTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT234743 WP_000450524.1 NZ_CP091773:1355282-1355509 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT234743 NZ_LR134079:c4974375-4974079 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A829CM33