Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 292352..292938 Replicon chromosome
Accession NZ_CP091773
Organism Escherichia coli strain SWYH.B349aT

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag L6V99_RS01320 Protein ID WP_042014162.1
Coordinates 292352..292582 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag L6V99_RS01325 Protein ID WP_000593555.1
Coordinates 292579..292938 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6V99_RS01310 (288389) 288389..291124 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
L6V99_RS01315 (291124) 291124..292248 + 1125 WP_001314210.1 ABC transporter permease -
L6V99_RS01320 (292352) 292352..292582 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
L6V99_RS01325 (292579) 292579..292938 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
L6V99_RS01330 (293058) 293058..293459 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
L6V99_RS01335 (293465) 293465..294271 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
L6V99_RS01340 (294268) 294268..295032 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
L6V99_RS01345 (295032) 295032..295865 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
L6V99_RS01350 (295862) 295862..296806 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T234736 WP_042014162.1 NZ_CP091773:292352-292582 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T234736 NZ_LR134079:3376863-3377150 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTGGATGATATAACTAGTTATATTGCCCGTTTCGACATAAA
TGCGGCAGAACGCTTATGGCAACGCTTAAGAGCTTGCGTCCTGCCGTTATCCGAACATCCGTATTTATACCCGCCAAGCG
ACAGAGTGCCAGGTTTACGAGAGATTGTGGCTCACCCCAATTACATAATTTTATACCGGGTGACAACTTCAGGTATTGAA
ATTGTAAACGTGGTTCACGCCCGCCGACAGTTCCCAATAACATCTTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT234736 WP_000593555.1 NZ_CP091773:292579-292938 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT234736 NZ_LR134079:3376671-3376856 [Escherichia coli]
ATGAATAGAGCTCTTTCACCAATGGTTTCTGAATTTGAAACCGTTGAGCAAGAAAACAGTTATAACGAATGGTTACGCGC
AAAAGTAGCCGCAAGCCTTGCTGATCCACGCCCGGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CCGAAATGCGCAAGGAACGGATATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References