Detailed information of TA system TA234730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2317442..2318417
• Replicon: chromosome
• Accession: NZ_CP091765
• Organism: Bacillus anthracis strain PNO2D1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: L6176_RS12095
• Protein ID: WP_002036394.1
• Coordinates: 2317442..2318179 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: L6176_RS12100
• Protein ID: WP_000588712.1
• Coordinates: 2318292..2318417 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6176_RS12075 (L6176_12075)	2313170..2313952	+	783	WP_000381787.1	class I SAM-dependent methyltransferase	-
L6176_RS12080 (L6176_12080)	2314074..2315759	-	1686	WP_014654562.1	alpha-keto acid decarboxylase family protein	-
L6176_RS12085 (L6176_12085)	2315866..2316348	+	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6176_RS12090 (L6176_12090)	2316516..2317253	+	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6176_RS12095 (L6176_12095)	2317442..2318179	+	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6176_RS12100 (L6176_12100)	2318292..2318417	+	126	WP_000588712.1	hypothetical protein	Antitoxin
L6176_RS12105 (L6176_12105)	2318494..2318670	+	177	WP_000852614.1	hypothetical protein	-
L6176_RS12110 (L6176_12110)	2318707..2318988	-	282	WP_001041555.1	hypothetical protein	-
L6176_RS12115 (L6176_12115)	2319265..2319501	-	237	WP_001021689.1	hypothetical protein	-
L6176_RS27890	2319638..2319763	+	126	WP_000694311.1	hypothetical protein	-
L6176_RS12120 (L6176_12120)	2319840..2320016	+	177	WP_000808045.1	stage II sporulation protein SB	-
L6176_RS12125 (L6176_12125)	2320160..2321629	+	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
L6176_RS12130 (L6176_12130)	2322193..2322663	-	471	WP_000670597.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T234730 WP_002036394.1 NZ_CP091765:2317442-2318179 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T234730 NZ_LR134079:c2521936-2521607 [Escherichia coli]
ATAAACTACATTATCGAATACTACAGTGACGAGGTTGAAGCTGAGATCCTTTCTCTTCCTGAGACCTTACAAGCCAGGTA
CATTCGGTACACGGAGAAAATGCGTATTTACGGAGCTAATCTTGGTTCCCCGCATACAGAGGCATTTGGCGACGGTTTGT
TTGAAATCCGCCTTAAGGGCTCGGAAGGCATAGGGCGTGTTTTTTACTGCACGCTAAAAGGAAAGCGAATCATTATGTTG
CATAGCTTTGTAAAGAAAACGCAAAAAACTCCACCTGCCGAGCTTAGAAAAGCTGAAACCAGAATGAAGGAGGTTAAGCA
TGACTGGTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT234730 WP_000588712.1 NZ_CP091765:2318292-2318417 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT234730 NZ_LR134079:c2521617-2521309 [Escherichia coli]
ATGACTGGTAAACGTACCCCACCTACCATGACACACGATGAAATGGTAGAAAAAATGTTGTCTAACCCCGCAGTAAAAGC
AGAATACGACGCCATCGCCGATGAATTCGCACTACTTGATGAAATGCTGGCAGCACGAAAAGAAGCTGGCTTAACCCAGG
CTGAAGTTGCTGAACGTATGGGAACAAAAGCGACTGCGATAACCAGAATGGAAAGCAATCTTGCATCAGGTACAAGCGGC
CCATCATTTGCCACACTGAAAAAATTCGCCCGTGCTACAGGGAAAAAACTCCAGATCCGCTTCGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1