Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241655..242297 Replicon chromosome
Accession NZ_CP091765
Organism Bacillus anthracis strain PNO2D1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag L6176_RS01340 Protein ID WP_000635963.1
Coordinates 241947..242297 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag L6176_RS01335 Protein ID WP_000004570.1
Coordinates 241655..241942 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6176_RS01310 (L6176_01310) 236971..237933 + 963 WP_000961037.1 UV DNA damage repair endonuclease UvsE -
L6176_RS01315 (L6176_01315) 237926..238498 - 573 WP_000906916.1 rhomboid family intramembrane serine protease -
L6176_RS01320 (L6176_01320) 238591..238950 + 360 WP_000635040.1 holo-ACP synthase -
L6176_RS01325 (L6176_01325) 239107..240057 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
L6176_RS01330 (L6176_01330) 240176..241345 + 1170 WP_000390596.1 alanine racemase -
L6176_RS01335 (L6176_01335) 241655..241942 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
L6176_RS01340 (L6176_01340) 241947..242297 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6176_RS01345 (L6176_01345) 242365..244533 + 2169 WP_000426225.1 Tex family protein -
L6176_RS01350 (L6176_01350) 244591..244707 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
L6176_RS01355 (L6176_01355) 244903..245361 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T234729 WP_000635963.1 NZ_CP091765:241947-242297 [Bacillus anthracis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T234729 NZ_LR134079:2244482-2244585 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT234729 WP_000004570.1 NZ_CP091765:241655-241942 [Bacillus anthracis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT234729 NZ_LR134079:c2244623-2244352 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References