Detailed information of TA system TA234728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2317415..2318390
• Replicon: chromosome
• Accession: NZ_CP091762
• Organism: Bacillus anthracis strain PNO2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A2R2IKP2
• Locus tag: L6174_RS12105
• Protein ID: WP_002036394.1
• Coordinates: 2317415..2318152 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: L6174_RS12110
• Protein ID: WP_000588712.1
• Coordinates: 2318265..2318390 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6174_RS12085 (L6174_12085)	2313143..2313925	+	783	WP_000381787.1	class I SAM-dependent methyltransferase	-
L6174_RS12090 (L6174_12090)	2314047..2315732	-	1686	WP_014654562.1	alpha-keto acid decarboxylase family protein	-
L6174_RS12095 (L6174_12095)	2315839..2316321	+	483	WP_000191911.1	MarR family winged helix-turn-helix transcriptional regulator	-
L6174_RS12100 (L6174_12100)	2316489..2317226	+	738	WP_000594139.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
L6174_RS12105 (L6174_12105)	2317415..2318152	+	738	WP_002036394.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6174_RS12110 (L6174_12110)	2318265..2318390	+	126	WP_000588712.1	hypothetical protein	Antitoxin
L6174_RS12115 (L6174_12115)	2318467..2318643	+	177	WP_000852614.1	hypothetical protein	-
L6174_RS12120 (L6174_12120)	2318680..2318961	-	282	WP_001041555.1	hypothetical protein	-
L6174_RS12125 (L6174_12125)	2319238..2319474	-	237	WP_001021689.1	hypothetical protein	-
L6174_RS28750	2319611..2319736	+	126	WP_000694311.1	hypothetical protein	-
L6174_RS12130 (L6174_12130)	2319813..2319989	+	177	WP_000808045.1	stage II sporulation protein SB	-
L6174_RS12135 (L6174_12135)	2320133..2321602	+	1470	WP_000287522.1	beta-Ala-His dipeptidase	-
L6174_RS12140 (L6174_12140)	2322166..2322636	-	471	WP_000670597.1	DUF5065 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28373.03 Da
• Isoelectric Point: 8.2705

>T234728 WP_002036394.1 NZ_CP091762:2317415-2318152 [Bacillus anthracis]
VISNIRIGLFILAIVFLVLVFFYWRNEELYEEKKQRIRKTWYGLFIVSVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T234728 NZ_LR134079:1501168-1501566 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT234728 WP_000588712.1 NZ_CP091762:2318265-2318390 [Bacillus anthracis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT234728 NZ_LR134079:1500941-1501168 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Structures

Toxin

• AlphaFold DB: A0A2R2IKP2

Antitoxin

• AlphaFold DB: A0A366GQT1