Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 9996..10521 Replicon plasmid p2-LC-1302-2020
Accession NZ_CP091758
Organism Escherichia coli strain LC-1302-2020

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag L6507_RS25985 Protein ID WP_001159871.1
Coordinates 9996..10301 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag L6507_RS25990 Protein ID WP_000813630.1
Coordinates 10303..10521 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6507_RS25970 (5952) 5952..7157 - 1206 WP_001442122.1 AAA family ATPase -
L6507_RS25975 (7778) 7778..8509 + 732 WP_000504263.1 replication initiation protein -
L6507_RS25980 (9189) 9189..9995 - 807 WP_000016968.1 site-specific integrase -
L6507_RS25985 (9996) 9996..10301 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
L6507_RS25990 (10303) 10303..10521 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L6507_RS25995 (11116) 11116..11385 + 270 WP_001554929.1 hypothetical protein -
L6507_RS26000 (11373) 11373..11969 + 597 WP_001554928.1 hypothetical protein -
L6507_RS26005 (11987) 11987..12334 - 348 WP_021573051.1 DUF6404 family protein -
L6507_RS26010 (12445) 12445..12792 + 348 WP_001554926.1 colicin E1 family microcin immunity protein -
L6507_RS26015 (12810) 12810..13100 - 291 WP_072184881.1 colicin-like pore-forming protein -
L6507_RS26020 (13448) 13448..13636 + 189 WP_000744815.1 hypothetical protein -
L6507_RS26025 (13764) 13764..15152 + 1389 WP_001554924.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..40699 40699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T234723 WP_001159871.1 NZ_CP091758:c10301-9996 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T234723 NZ_LR134079:335375-335902 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTCACTCTTGGGCGTCTGGCAATTGATCATTCAATACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT234723 WP_000813630.1 NZ_CP091758:c10521-10303 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT234723 NZ_LR134079:335102-335368 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATTCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGTAAAACGTCTTCAGGGAATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References