Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 255962..256605 Replicon plasmid p1-LC-1302-2020-KPC3
Accession NZ_CP091757
Organism Escherichia coli strain LC-1302-2020

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag L6507_RS25740 Protein ID WP_001044770.1
Coordinates 256189..256605 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag L6507_RS25735 Protein ID WP_001261282.1
Coordinates 255962..256192 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6507_RS25695 (L6507_25695) 251160..251633 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
L6507_RS25700 (L6507_25700) 251725..251955 + 231 WP_011977773.1 hypothetical protein -
L6507_RS25705 (L6507_25705) 252847..253629 - 783 WP_004152340.1 site-specific integrase -
L6507_RS25710 (L6507_25710) 253629..253961 - 333 WP_004152339.1 hypothetical protein -
L6507_RS25715 (L6507_25715) 253968..254366 - 399 WP_004171440.1 hypothetical protein -
L6507_RS25720 (L6507_25720) 254392..254721 - 330 WP_004152337.1 hypothetical protein -
L6507_RS25725 (L6507_25725) 254749..255057 - 309 WP_004152336.1 hypothetical protein -
L6507_RS25730 (L6507_25730) 255544..256005 - 462 WP_072093212.1 hypothetical protein -
L6507_RS25735 (L6507_25735) 255962..256192 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6507_RS25740 (L6507_25740) 256189..256605 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
L6507_RS25745 (L6507_25745) 256679..257368 + 690 Protein_290 ATP-binding protein -
L6507_RS25750 (L6507_25750) 257423..258120 + 698 WP_223174630.1 IS1 family transposase -
L6507_RS25755 (L6507_25755) 258242..258472 - 231 Protein_292 IS5/IS1182 family transposase -
L6507_RS25760 (L6507_25760) 258574..259758 + 1185 WP_241450021.1 ISAs1-like element ISKpn31 family transposase -
L6507_RS25765 (L6507_25765) 260035..261354 + 1320 WP_004152397.1 IS1182-like element ISKpn6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / tet(A) / qnrB1 / dfrA14 / blaKPC-3 - 1..298935 298935
- flank IS/Tn - - 249772..251040 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T234722 WP_001044770.1 NZ_CP091757:256189-256605 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T234722 NZ_LR134079:292671-292946 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT234722 WP_001261282.1 NZ_CP091757:255962-256192 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT234722 NZ_LR134079:292943-293302 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References