Detailed information of TA system TA234721

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53673..54409
• Replicon: plasmid p1-LC-1302-2020-KPC3
• Accession: NZ_CP091757
• Organism: Escherichia coli strain LC-1302-2020

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: L6507_RS24575
• Protein ID: WP_003026803.1
• Coordinates: 53927..54409 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: L6507_RS24570
• Protein ID: WP_003026799.1
• Coordinates: 53673..53939 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6507_RS24525 (L6507_24525)	49735..50097	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
L6507_RS24530 (L6507_24530)	50147..50497	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
L6507_RS24535 (L6507_24535)	50855..51124	+	270	WP_004152102.1	hypothetical protein	-
L6507_RS24540 (L6507_24540)	51112..51687	+	576	WP_004152103.1	hypothetical protein	-
L6507_RS24545 (L6507_24545)	51718..52212	+	495	WP_004152104.1	DNA-binding protein	-
L6507_RS24550 (L6507_24550)	52256..52624	+	369	WP_004152105.1	hypothetical protein	-
L6507_RS24555 (L6507_24555)	52658..52861	+	204	WP_004152106.1	HHA domain-containing protein	-
L6507_RS24560 (L6507_24560)	52910..53167	+	258	WP_004152107.1	hypothetical protein	-
L6507_RS24565 (L6507_24565)	53243..53497	+	255	WP_004152108.1	hypothetical protein	-
L6507_RS24570 (L6507_24570)	53673..53939	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
L6507_RS24575 (L6507_24575)	53927..54409	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
L6507_RS24580 (L6507_24580)	54617..55963	+	1347	WP_077253535.1	ISNCY family transposase	-
L6507_RS24585 (L6507_24585)	56012..56407	+	396	WP_004143398.1	helix-turn-helix domain-containing protein	-
L6507_RS24590 (L6507_24590)	56555..57720	-	1166	Protein_59	IS3 family transposase	-
L6507_RS24595 (L6507_24595)	57897..58859	-	963	WP_004152113.1	zinc metalloprotease HtpX	-
L6507_RS24600 (L6507_24600)	58846..59334	-	489	WP_004152114.1	phosphate-starvation-inducible PsiE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / tet(A) / qnrB1 / dfrA14 / blaKPC-3	-	1..298935	298935

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T234721 WP_003026803.1 NZ_CP091757:53927-54409 [Escherichia coli]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T234721 NZ_LR134079:209301-209408 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT234721 WP_003026799.1 NZ_CP091757:53673-53939 [Escherichia coli]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT234721 NZ_LR134079:c209244-209187 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66