Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3655106..3655724 Replicon chromosome
Accession NZ_CP091756
Organism Escherichia coli strain LC-1302-2020

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L6507_RS17775 Protein ID WP_001291435.1
Coordinates 3655506..3655724 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L6507_RS17770 Protein ID WP_000344800.1
Coordinates 3655106..3655480 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6507_RS17760 (3650195) 3650195..3651388 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6507_RS17765 (3651411) 3651411..3654560 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L6507_RS17770 (3655106) 3655106..3655480 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L6507_RS17775 (3655506) 3655506..3655724 + 219 WP_001291435.1 HHA domain-containing protein Toxin
L6507_RS17780 (3655896) 3655896..3656447 + 552 WP_000102564.1 maltose O-acetyltransferase -
L6507_RS17785 (3656563) 3656563..3657033 + 471 WP_000136192.1 YlaC family protein -
L6507_RS17790 (3657197) 3657197..3658747 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L6507_RS17795 (3658789) 3658789..3659142 - 354 WP_000878140.1 DUF1428 domain-containing protein -
L6507_RS17805 (3659521) 3659521..3659832 + 312 WP_000409911.1 MGMT family protein -
L6507_RS17810 (3659863) 3659863..3660435 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T234716 WP_001291435.1 NZ_CP091756:3655506-3655724 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T234716 NZ_LR134075:3599005-3599283 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT234716 WP_000344800.1 NZ_CP091756:3655106-3655480 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT234716 NZ_LR134075:3598742-3599002 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References