Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2427536..2428062 Replicon chromosome
Accession NZ_CP091756
Organism Escherichia coli strain LC-1302-2020

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag L6507_RS11720 Protein ID WP_000323025.1
Coordinates 2427775..2428062 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag L6507_RS11715 Protein ID WP_000534858.1
Coordinates 2427536..2427775 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6507_RS11685 (2423400) 2423400..2423651 - 252 WP_000005552.1 excisionase family protein -
L6507_RS11690 (2423724) 2423724..2424464 - 741 Protein_2286 3'-5' exoribonuclease -
L6507_RS11700 (2426085) 2426085..2426234 + 150 WP_011443592.1 protein YdfW -
L6507_RS11705 (2426671) 2426671..2427003 - 333 WP_001301033.1 FlxA-like family protein -
L6507_RS11710 (2427206) 2427206..2427511 - 306 WP_001326990.1 protein YdfV -
L6507_RS11715 (2427536) 2427536..2427775 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
L6507_RS11720 (2427775) 2427775..2428062 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
L6507_RS11725 (2428134) 2428134..2428289 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
L6507_RS11730 (2428506) 2428506..2428757 + 252 WP_000980994.1 protein Rem -
L6507_RS11735 (2428824) 2428824..2429102 + 279 WP_032351280.1 hypothetical protein -
L6507_RS11740 (2429104) 2429104..2430153 + 1050 WP_001265199.1 DUF968 domain-containing protein -
L6507_RS11745 (2430167) 2430167..2430304 + 138 Protein_2297 antitermination protein -
L6507_RS11750 (2430359) 2430359..2431572 + 1214 WP_136952279.1 IS3 family transposase -
L6507_RS11755 (2431612) 2431612..2432232 + 621 Protein_2299 antitermination protein -
L6507_RS11760 (2432510) 2432510..2432599 - 90 WP_120795389.1 hypothetical protein -
L6507_RS11765 (2432654) 2432654..2432866 - 213 WP_000087756.1 cold shock-like protein CspF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2407790..2464627 56837
- inside IScluster/Tn - - 2419357..2431572 12215


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T234713 WP_000323025.1 NZ_CP091756:2427775-2428062 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T234713 NZ_LR134075:2570051-2570158 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT234713 WP_000534858.1 NZ_CP091756:2427536-2427775 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT234713 NZ_LR134075:c2570004-2569938 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References