Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 209946..210589 Replicon plasmid p1-LC-1303-2020-KPC3
Accession NZ_CP091753
Organism Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag L6506_RS27855 Protein ID WP_001044770.1
Coordinates 210173..210589 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag L6506_RS27850 Protein ID WP_001261282.1
Coordinates 209946..210176 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6506_RS27810 (L6506_27810) 205144..205617 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
L6506_RS27815 (L6506_27815) 205709..205939 + 231 WP_011977773.1 hypothetical protein -
L6506_RS27820 (L6506_27820) 206831..207613 - 783 WP_004152340.1 site-specific integrase -
L6506_RS27825 (L6506_27825) 207613..207945 - 333 WP_004152339.1 hypothetical protein -
L6506_RS27830 (L6506_27830) 207952..208350 - 399 WP_004171440.1 hypothetical protein -
L6506_RS27835 (L6506_27835) 208376..208705 - 330 WP_004152337.1 hypothetical protein -
L6506_RS27840 (L6506_27840) 208733..209041 - 309 WP_004152336.1 hypothetical protein -
L6506_RS27845 (L6506_27845) 209528..209989 - 462 WP_072093212.1 hypothetical protein -
L6506_RS27850 (L6506_27850) 209946..210176 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6506_RS27855 (L6506_27855) 210173..210589 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
L6506_RS27860 (L6506_27860) 210663..211352 + 690 Protein_238 ATP-binding protein -
L6506_RS27865 (L6506_27865) 211407..212104 + 698 WP_223174630.1 IS1 family transposase -
L6506_RS27870 (L6506_27870) 212226..212456 - 231 Protein_240 IS5/IS1182 family transposase -
L6506_RS27875 (L6506_27875) 212558..213742 + 1185 WP_241450021.1 ISAs1-like element ISKpn31 family transposase -
L6506_RS27880 (L6506_27880) 214019..215338 + 1320 WP_004152397.1 IS1182-like element ISKpn6 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / tet(A) / qnrB1 / dfrA14 / blaKPC-3 - 1..252919 252919
- flank IS/Tn - - 203756..205024 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T234698 WP_001044770.1 NZ_CP091753:210173-210589 [Klebsiella grimontii]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T234698 NZ_LR134031:c4748420-4748109 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT234698 WP_001261282.1 NZ_CP091753:209946-210176 [Klebsiella grimontii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT234698 NZ_LR134031:c4748108-4747818 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References