Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53673..54409 Replicon plasmid p1-LC-1303-2020-KPC3
Accession NZ_CP091753
Organism Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag L6506_RS26950 Protein ID WP_003026803.1
Coordinates 53927..54409 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag L6506_RS26945 Protein ID WP_003026799.1
Coordinates 53673..53939 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6506_RS26900 (L6506_26900) 49735..50097 - 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -
L6506_RS26905 (L6506_26905) 50147..50497 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
L6506_RS26910 (L6506_26910) 50855..51124 + 270 WP_004152102.1 hypothetical protein -
L6506_RS26915 (L6506_26915) 51112..51687 + 576 WP_004152103.1 hypothetical protein -
L6506_RS26920 (L6506_26920) 51718..52212 + 495 WP_004152104.1 DNA-binding protein -
L6506_RS26925 (L6506_26925) 52256..52624 + 369 WP_004152105.1 hypothetical protein -
L6506_RS26930 (L6506_26930) 52658..52861 + 204 WP_004152106.1 HHA domain-containing protein -
L6506_RS26935 (L6506_26935) 52910..53167 + 258 WP_004152107.1 hypothetical protein -
L6506_RS26940 (L6506_26940) 53243..53497 + 255 WP_004152108.1 hypothetical protein -
L6506_RS26945 (L6506_26945) 53673..53939 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
L6506_RS26950 (L6506_26950) 53927..54409 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
L6506_RS26955 (L6506_26955) 54617..55963 + 1347 WP_077253535.1 ISNCY family transposase -
L6506_RS26960 (L6506_26960) 56012..56407 + 396 WP_004143398.1 helix-turn-helix domain-containing protein -
L6506_RS26965 (L6506_26965) 56555..57720 - 1166 Protein_59 IS3 family transposase -
L6506_RS26970 (L6506_26970) 57897..58859 - 963 WP_004152113.1 zinc metalloprotease HtpX -
L6506_RS26975 (L6506_26975) 58846..59334 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / blaCTX-M-15 / aac(3)-IIa / aac(6')-Ib-cr / tet(A) / qnrB1 / dfrA14 / blaKPC-3 - 1..252919 252919


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T234697 WP_003026803.1 NZ_CP091753:53927-54409 [Klebsiella grimontii]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T234697 NZ_LR134031:c4442312-4442139 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT234697 WP_003026799.1 NZ_CP091753:53673-53939 [Klebsiella grimontii]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT234697 NZ_LR134031:c4442636-4442340 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References