Detailed information of TA system TA234696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5610803..5611413
• Replicon: chromosome
• Accession: NZ_CP091752
• Organism: Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: L6506_RS26270
• Protein ID: WP_098363641.1
• Coordinates: 5610803..5611177 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: L6506_RS26275
• Protein ID: WP_077253475.1
• Coordinates: 5611174..5611413 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6506_RS26255 (L6506_26255)	5607958..5608860	+	903	WP_004122548.1	formate dehydrogenase subunit beta	-
L6506_RS26260 (L6506_26260)	5608857..5609492	+	636	WP_004132860.1	formate dehydrogenase cytochrome b556 subunit	-
L6506_RS26265 (L6506_26265)	5609836..5610765	+	930	WP_004132861.1	formate dehydrogenase accessory protein FdhE	-
L6506_RS26270 (L6506_26270)	5610803..5611177	-	375	WP_098363641.1	type II toxin-antitoxin system VapC family toxin	Toxin
L6506_RS26275 (L6506_26275)	5611174..5611413	-	240	WP_077253475.1	CopG family transcriptional regulator	Antitoxin
L6506_RS26280 (L6506_26280)	5611619..5612536	+	918	WP_024360706.1	alpha/beta hydrolase	-
L6506_RS26285 (L6506_26285)	5612554..5613495	-	942	WP_004127112.1	fatty acid biosynthesis protein FabY	-
L6506_RS26290 (L6506_26290)	5613540..5613977	-	438	WP_004132870.1	D-aminoacyl-tRNA deacylase	-
L6506_RS26295 (L6506_26295)	5613974..5614834	-	861	WP_004127107.1	virulence factor BrkB family protein	-
L6506_RS26300 (L6506_26300)	5614828..5615427	-	600	WP_024360709.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-110)

Antitoxin

• Ribbon-helix-helix protein, CopG (13-47)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14081.36 Da
• Isoelectric Point: 8.4571

>T234696 WP_098363641.1 NZ_CP091752:c5611177-5610803 [Klebsiella grimontii]
MIKGPALFDTNILIDLFSGRVEAKQVLEAYPPQNAISLITWTEVMVGAKKYHQEHRTRIALSAFNIIDISQDIAERSVNL
RKEYGMKLPDAIILATAQIHRYELVTRNTKDFFGIPGVITPYHL

Nucleotide

• Length: 375 bp

>T234696 NZ_LR134031:4215952-4216209 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9293.49 Da
• Isoelectric Point: 4.3142

>AT234696 WP_077253475.1 NZ_CP091752:c5611413-5611174 [Klebsiella grimontii]
MMTGTNMGRILLDLSDDVIKRLEDLKQQRNLPRAELLREAVEQYLERQADTTISNALGIWQGCEEDGVEFERKLREEWQ

Nucleotide

• Length: 240 bp

>AT234696 NZ_LR134031:4216221-4216766 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCGGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCTTTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA