Detailed information of TA system TA234695

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 5031023..5031713
• Replicon: chromosome
• Accession: NZ_CP091752
• Organism: Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: L6506_RS23555
• Protein ID: WP_041165267.1
• Coordinates: 5031023..5031364 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: L6506_RS23560
• Protein ID: WP_224253094.1
• Coordinates: 5031387..5031713 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6506_RS23530 (L6506_23530)	5026753..5027076	+	324	WP_154063373.1	endoribonuclease SymE	-
L6506_RS23535 (L6506_23535)	5027223..5027657	+	435	WP_049113956.1	VOC family protein	-
L6506_RS23540 (L6506_23540)	5027823..5028566	+	744	WP_224253091.1	hypothetical protein	-
L6506_RS23545 (L6506_23545)	5028876..5029871	-	996	WP_224253092.1	hypothetical protein	-
L6506_RS23550 (L6506_23550)	5030075..5030908	-	834	WP_224253093.1	DUF4942 domain-containing protein	-
L6506_RS23555 (L6506_23555)	5031023..5031364	-	342	WP_041165267.1	TA system toxin CbtA family protein	Toxin
L6506_RS23560 (L6506_23560)	5031387..5031713	-	327	WP_224253094.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L6506_RS23565 (L6506_23565)	5031727..5032203	-	477	WP_224253095.1	DNA repair protein RadC	-
L6506_RS23570 (L6506_23570)	5032214..5032660	-	447	WP_014227715.1	antirestriction protein	-
L6506_RS23575 (L6506_23575)	5032884..5033573	-	690	WP_041165265.1	hypothetical protein	-
L6506_RS23580 (L6506_23580)	5034137..5035027	-	891	WP_014227713.1	YfjP family GTPase	-
L6506_RS23585 (L6506_23585)	5035110..5036198	-	1089	WP_224253096.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	5026753..5055119	28366

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (8-106)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12768.64 Da
• Isoelectric Point: 7.9093

>T234695 WP_041165267.1 NZ_CP091752:c5031364-5031023 [Klebsiella grimontii]
MKTLPATTQRAAKPCPSPVTVWQMLLTRLLEQHYGLTLNDTPFSDETVIQEHINAGITLADAVNFLVEKYELIRIDRRGF
NCQEQSPYLGAADILRARQATGLLRQSHHPSIR

Nucleotide

• Length: 342 bp

>T234695 NZ_LR134031:4178765-4179106 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCACTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12310.07 Da
• Isoelectric Point: 6.9563

>AT234695 WP_224253094.1 NZ_CP091752:c5031713-5031387 [Klebsiella grimontii]
MPLTDGIQWGLKRSFTPNFGARLVQEGNRLHNLADRANITGKFVDVECQKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPIQR

Nucleotide

• Length: 327 bp

>AT234695 NZ_LR134031:c4178770-4178694 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT