Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4304859..4305478 Replicon chromosome
Accession NZ_CP091752
Organism Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag L6506_RS20155 Protein ID WP_004099646.1
Coordinates 4305260..4305478 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag L6506_RS20150 Protein ID WP_004129911.1
Coordinates 4304859..4305233 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6506_RS20140 (L6506_20140) 4300028..4301221 + 1194 WP_138110236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6506_RS20145 (L6506_20145) 4301244..4304390 + 3147 WP_004848339.1 multidrug efflux RND transporter permease subunit AcrB -
L6506_RS20150 (L6506_20150) 4304859..4305233 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
L6506_RS20155 (L6506_20155) 4305260..4305478 + 219 WP_004099646.1 HHA domain-containing protein Toxin
L6506_RS20160 (L6506_20160) 4305640..4306206 + 567 WP_004136050.1 maltose O-acetyltransferase -
L6506_RS20165 (L6506_20165) 4306178..4306312 - 135 WP_223226911.1 hypothetical protein -
L6506_RS20170 (L6506_20170) 4306333..4306803 + 471 WP_004136048.1 YlaC family protein -
L6506_RS20175 (L6506_20175) 4306778..4308232 - 1455 WP_224246708.1 PLP-dependent aminotransferase family protein -
L6506_RS20180 (L6506_20180) 4308333..4309031 + 699 WP_004136044.1 GNAT family protein -
L6506_RS20185 (L6506_20185) 4309028..4309168 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
L6506_RS20190 (L6506_20190) 4309168..4309431 - 264 WP_004129897.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T234692 WP_004099646.1 NZ_CP091752:4305260-4305478 [Klebsiella grimontii]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T234692 NZ_LR134031:c3784044-3783646 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT234692 WP_004129911.1 NZ_CP091752:4304859-4305233 [Klebsiella grimontii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT234692 NZ_LR134031:c3784340-3784047 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References