Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 241856..242498 Replicon chromosome
Accession NC_011773
Organism Bacillus cereus AH820

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag BCAH820_RS01360 Protein ID WP_000635963.1
Coordinates 242148..242498 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCAH820_RS01355 Protein ID WP_000004570.1
Coordinates 241856..242143 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCAH820_RS01330 237172..238134 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
BCAH820_RS01335 238127..238699 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
BCAH820_RS01340 238792..239151 + 360 WP_000635040.1 holo-ACP synthase -
BCAH820_RS01345 239308..240258 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
BCAH820_RS01350 240377..241546 + 1170 WP_000390600.1 alanine racemase -
BCAH820_RS01355 241856..242143 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCAH820_RS01360 242148..242498 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCAH820_RS01365 242566..244734 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
BCAH820_RS01370 244792..244908 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
BCAH820_RS01375 245104..245562 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T23469 WP_000635963.1 NC_011773:242148-242498 [Bacillus cereus AH820]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T23469 NC_011773:242148-242498 [Bacillus cereus AH820]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT23469 WP_000004570.1 NC_011773:241856-242143 [Bacillus cereus AH820]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT23469 NC_011773:241856-242143 [Bacillus cereus AH820]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References