Detailed information of TA system TA234681

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 830898..831555
• Replicon: chromosome
• Accession: NZ_CP091752
• Organism: Klebsiella grimontii strain LC-1303-2020

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A9E1DA00
• Locus tag: L6506_RS04030
• Protein ID: WP_024358614.1
• Coordinates: 831145..831555 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: H3N295
• Locus tag: L6506_RS04025
• Protein ID: WP_004124953.1
• Coordinates: 830898..831164 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6506_RS04000 (L6506_04000)	826076..827509	-	1434	WP_024358622.1	6-phospho-beta-glucosidase	-
L6506_RS04005 (L6506_04005)	827631..828359	-	729	WP_024358621.1	MurR/RpiR family transcriptional regulator	-
L6506_RS04010 (L6506_04010)	828410..828721	+	312	WP_004133599.1	N(4)-acetylcytidine aminohydrolase	-
L6506_RS04015 (L6506_04015)	828884..829543	+	660	WP_004124978.1	hemolysin III family protein	-
L6506_RS04020 (L6506_04020)	829670..830653	-	984	WP_241449745.1	tRNA-modifying protein YgfZ	-
L6506_RS04025 (L6506_04025)	830898..831164	+	267	WP_004124953.1	FAD assembly factor SdhE	Antitoxin
L6506_RS04030 (L6506_04030)	831145..831555	+	411	WP_024358614.1	protein YgfX	Toxin
L6506_RS04035 (L6506_04035)	831564..832085	-	522	WP_024358613.1	flavodoxin FldB	-
L6506_RS04040 (L6506_04040)	832207..833103	+	897	WP_004124942.1	site-specific tyrosine recombinase XerD	-
L6506_RS04045 (L6506_04045)	833126..833839	+	714	WP_241449746.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
L6506_RS04050 (L6506_04050)	833845..835578	+	1734	WP_098066860.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16135.97 Da
• Isoelectric Point: 10.9455

>T234681 WP_024358614.1 NZ_CP091752:831145-831555 [Klebsiella grimontii]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWEIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLQKPTQE

Nucleotide

• Length: 411 bp

>T234681 NZ_LR134031:c832183-831806 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10411.88 Da
• Isoelectric Point: 5.1296

>AT234681 WP_004124953.1 NZ_CP091752:830898-831164 [Klebsiella grimontii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT234681 NZ_LR134031:c832607-832230 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3H3L9