Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3785201..3785855 Replicon chromosome
Accession NZ_CP091712
Organism Escherichia coli strain KFu014

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag L6L57_RS18010 Protein ID WP_000244772.1
Coordinates 3785201..3785608 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag L6L57_RS18015 Protein ID WP_000354046.1
Coordinates 3785589..3785855 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L57_RS17990 (3781158) 3781158..3782891 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
L6L57_RS17995 (3782897) 3782897..3783607 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
L6L57_RS18000 (3783632) 3783632..3784528 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
L6L57_RS18005 (3784640) 3784640..3785161 + 522 WP_001055874.1 flavodoxin FldB -
L6L57_RS18010 (3785201) 3785201..3785608 - 408 WP_000244772.1 protein YgfX Toxin
L6L57_RS18015 (3785589) 3785589..3785855 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
L6L57_RS18020 (3786098) 3786098..3787078 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
L6L57_RS18025 (3787155) 3787155..3787814 - 660 WP_000250274.1 hemolysin III family protein -
L6L57_RS18030 (3787978) 3787978..3788289 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
L6L57_RS18035 (3788334) 3788334..3789767 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
L6L57_RS18040 (3789824) 3789824..3790567 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T234677 WP_000244772.1 NZ_CP091712:c3785608-3785201 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T234677 NZ_LR134000:c4179213-4179040 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT234677 WP_000354046.1 NZ_CP091712:c3785855-3785589 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT234677 NZ_LR134000:c4179537-4179241 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References