Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3643398..3643981 Replicon chromosome
Accession NZ_CP091712
Organism Escherichia coli strain KFu014

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag L6L57_RS17370 Protein ID WP_000254750.1
Coordinates 3643398..3643733 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag L6L57_RS17375 Protein ID WP_000581937.1
Coordinates 3643733..3643981 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L57_RS17355 (3639285) 3639285..3640583 - 1299 WP_000036723.1 phosphopyruvate hydratase -
L6L57_RS17360 (3640670) 3640670..3642307 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
L6L57_RS17365 (3642535) 3642535..3643326 - 792 WP_001071641.1 nucleoside triphosphate pyrophosphohydrolase -
L6L57_RS17370 (3643398) 3643398..3643733 - 336 WP_000254750.1 endoribonuclease MazF Toxin
L6L57_RS17375 (3643733) 3643733..3643981 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
L6L57_RS17380 (3644059) 3644059..3646293 - 2235 WP_000226795.1 GTP diphosphokinase -
L6L57_RS17385 (3646341) 3646341..3647642 - 1302 WP_000046808.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3637902..3639230 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T234676 WP_000254750.1 NZ_CP091712:c3643733-3643398 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T234676 NZ_LR134000:c4004154-4003777 [Escherichia coli]
ATGAAAACATTACCTGATACACACGTACGGAAGGTATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT234676 WP_000581937.1 NZ_CP091712:c3643981-3643733 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT234676 NZ_LR134000:c4004611-4004243 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAATTAATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCTTTTTATCCAACATCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References