Detailed information of TA system TA234674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2398466..2399104
• Replicon: chromosome
• Accession: NZ_CP091712
• Organism: Escherichia coli strain KFu014

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: L6L57_RS11495
• Protein ID: WP_000813794.1
• Coordinates: 2398928..2399104 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: L6L57_RS11490
• Protein ID: WP_001270286.1
• Coordinates: 2398466..2398882 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L57_RS11470 (2393618)	2393618..2394559	-	942	WP_001251303.1	ABC transporter permease	-
L6L57_RS11475 (2394560)	2394560..2395573	-	1014	WP_000220391.1	ABC transporter ATP-binding protein	-
L6L57_RS11480 (2395591)	2395591..2396736	-	1146	WP_000034364.1	ABC transporter substrate-binding protein	-
L6L57_RS11485 (2396981)	2396981..2398387	-	1407	WP_000760611.1	PLP-dependent aminotransferase family protein	-
L6L57_RS11490 (2398466)	2398466..2398882	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
L6L57_RS11495 (2398928)	2398928..2399104	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
L6L57_RS11500 (2399326)	2399326..2399556	+	231	WP_000491567.1	DUF2554 family protein	-
L6L57_RS11505 (2399648)	2399648..2401609	-	1962	WP_012311648.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
L6L57_RS11510 (2401682)	2401682..2402218	-	537	WP_000429154.1	DNA-binding transcriptional regulator SutR	-
L6L57_RS11515 (2402310)	2402310..2403191	+	882	Protein_2253	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2403206..2404534	1328

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T234674 WP_000813794.1 NZ_CP091712:c2399104-2398928 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T234674 NZ_LR134000:c3935370-3935083 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT234674 WP_001270286.1 NZ_CP091712:c2398882-2398466 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT234674 NZ_LR134000:c3935096-3934794 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• PDB: 6HPB