23467

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 231676..232318 Replicon chromosome
Accession NC_011772
Organism Bacillus cereus G9842

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag BCG9842_RS01270 Protein ID WP_000635965.1
Coordinates 231968..232318 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag BCG9842_RS01265 Protein ID WP_000004570.1
Coordinates 231676..231963 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BCG9842_RS01240 226991..227953 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
BCG9842_RS01245 227946..228518 - 573 WP_000908527.1 rhomboid family intramembrane serine protease -
BCG9842_RS01250 228612..228971 + 360 WP_000583423.1 holo-ACP synthase -
BCG9842_RS01255 229128..230078 + 951 WP_002084009.1 outer membrane lipoprotein carrier protein LolA -
BCG9842_RS01260 230196..231365 + 1170 WP_000390595.1 alanine racemase -
BCG9842_RS01265 231676..231963 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
BCG9842_RS01270 231968..232318 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
BCG9842_RS01275 232386..234554 + 2169 WP_000426212.1 RNA-binding transcriptional accessory protein -
BCG9842_RS29060 234612..234728 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
BCG9842_RS01280 234924..235382 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T23467 WP_000635965.1 NC_011772:231968-232318 [Bacillus cereus G9842]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T23467 NC_011772:231968-232318 [Bacillus cereus G9842]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT23467 WP_000004570.1 NC_011772:231676-231963 [Bacillus cereus G9842]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT23467 NC_011772:231676-231963 [Bacillus cereus G9842]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References