Detailed information of TA system TA234665

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1538856..1539561
• Replicon: chromosome
• Accession: NZ_CP091712
• Organism: Escherichia coli strain KFu014

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: L6L57_RS07260
• Protein ID: WP_000539521.1
• Coordinates: 1539175..1539561 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6L57_RS07255
• Protein ID: WP_001280945.1
• Coordinates: 1538856..1539185 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L57_RS07240 (1534014)	1534014..1534925	+	912	WP_001236058.1	glutathione ABC transporter permease GsiD	-
L6L57_RS07245 (1535102)	1535102..1537450	+	2349	WP_000950337.1	EAL domain-containing protein	-
L6L57_RS07250 (1537458)	1537458..1538786	+	1329	WP_000086869.1	GGDEF domain-containing protein	-
L6L57_RS07255 (1538856)	1538856..1539185	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
L6L57_RS07260 (1539175)	1539175..1539561	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6L57_RS07265 (1539787)	1539787..1541112	-	1326	WP_240724727.1	30S ribosomal protein S12 methylthiotransferase RimO	-
L6L57_RS07270 (1541325)	1541325..1541708	+	384	WP_000497137.1	biofilm formation regulator BssR	-
L6L57_RS07275 (1541819)	1541819..1542934	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
L6L57_RS07280 (1542931)	1542931..1543557	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T234665 WP_000539521.1 NZ_CP091712:c1539561-1539175 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T234665 NZ_LR134000:2257557-2257844 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT234665 WP_001280945.1 NZ_CP091712:c1539185-1538856 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT234665 NZ_LR134000:2257318-2257557 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6