Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1184212..1184830 Replicon chromosome
Accession NZ_CP091712
Organism Escherichia coli strain KFu014

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag L6L57_RS05545 Protein ID WP_001291435.1
Coordinates 1184212..1184430 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag L6L57_RS05550 Protein ID WP_000344800.1
Coordinates 1184456..1184830 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L57_RS05510 (1179502) 1179502..1180074 + 573 WP_000779831.1 YbaY family lipoprotein -
L6L57_RS05515 (1180105) 1180105..1180416 - 312 WP_000409911.1 MGMT family protein -
L6L57_RS05525 (1180795) 1180795..1181148 + 354 WP_000878140.1 DUF1428 domain-containing protein -
L6L57_RS05530 (1181189) 1181189..1182739 - 1551 WP_012311818.1 cyclic-guanylate-specific phosphodiesterase PdeB -
L6L57_RS05535 (1182903) 1182903..1183373 - 471 WP_000136192.1 YlaC family protein -
L6L57_RS05540 (1183489) 1183489..1184040 - 552 WP_000102561.1 maltose O-acetyltransferase -
L6L57_RS05545 (1184212) 1184212..1184430 - 219 WP_001291435.1 HHA domain-containing protein Toxin
L6L57_RS05550 (1184456) 1184456..1184830 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
L6L57_RS05555 (1185376) 1185376..1188525 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
L6L57_RS05560 (1188548) 1188548..1189741 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T234664 WP_001291435.1 NZ_CP091712:c1184430-1184212 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T234664 NZ_LR134000:1979412-1979515 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT234664 WP_000344800.1 NZ_CP091712:c1184830-1184456 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT234664 NZ_LR134000:c1979553-1979282 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References