Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1149904..1150741 Replicon chromosome
Accession NZ_CP091712
Organism Escherichia coli strain KFu014

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag L6L57_RS05375 Protein ID WP_000227787.1
Coordinates 1149904..1150446 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag L6L57_RS05380 Protein ID WP_001353405.1
Coordinates 1150430..1150741 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L57_RS05355 (1145444) 1145444..1146355 - 912 WP_000705871.1 2-dehydropantoate 2-reductase -
L6L57_RS05360 (1146523) 1146523..1147014 + 492 WP_001138904.1 nucleotide binding protein YajQ -
L6L57_RS05365 (1147142) 1147142..1148506 - 1365 WP_001000978.1 MFS transporter -
L6L57_RS05370 (1148913) 1148913..1149848 + 936 WP_012311701.1 tetratricopeptide repeat protein -
L6L57_RS05375 (1149904) 1149904..1150446 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
L6L57_RS05380 (1150430) 1150430..1150741 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
L6L57_RS05385 (1150926) 1150926..1151816 - 891 WP_000971336.1 heme o synthase -
L6L57_RS05390 (1151828) 1151828..1152157 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
L6L57_RS05395 (1152157) 1152157..1152771 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
L6L57_RS05400 (1152761) 1152761..1154752 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
L6L57_RS05405 (1154774) 1154774..1155721 - 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T234663 WP_000227787.1 NZ_CP091712:c1150446-1149904 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T234663 NZ_LR134000:988358-988693 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT234663 WP_001353405.1 NZ_CP091712:c1150741-1150430 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT234663 NZ_LR134000:988110-988358 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCAGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References