Detailed information of TA system TA234662

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 995010..995716
• Replicon: chromosome
• Accession: NZ_CP091712
• Organism: Escherichia coli strain KFu014

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: V0SXW1
• Locus tag: L6L57_RS04635
• Protein ID: WP_000854677.1
• Coordinates: 995348..995716 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: V0SU84
• Locus tag: L6L57_RS04630
• Protein ID: WP_000065824.1
• Coordinates: 995010..995327 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6L57_RS04600 (991854)	991854..992005	+	152	Protein_894	IS3 family transposase	-
L6L57_RS04605 (992101)	992101..993278	+	1178	WP_152895938.1	IS3 family transposase	-
L6L57_RS04610 (993489)	993489..993725	+	237	Protein_896	DUF905 domain-containing protein	-
L6L57_RS04615 (993811)	993811..994269	+	459	WP_000211834.1	antirestriction protein	-
L6L57_RS04620 (994281)	994281..994760	+	480	WP_000438147.1	RadC family protein	-
L6L57_RS04625 (994769)	994769..994990	+	222	WP_001220307.1	DUF987 domain-containing protein	-
L6L57_RS04630 (995010)	995010..995327	+	318	WP_000065824.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
L6L57_RS04635 (995348)	995348..995716	+	369	WP_000854677.1	TA system toxin CbtA family protein	Toxin
L6L57_RS04640 (996509)	996509..997123	+	615	WP_001019920.1	YagU family protein	-
L6L57_RS04645 (997372)	997372..997701	-	330	WP_001303809.1	YkgJ family cysteine cluster protein	-
L6L57_RS04650 (998014)	998014..998724	-	711	WP_001361803.1	fimbrial chaperone EcpE	-
L6L57_RS04655 (998693)	998693..1000336	-	1644	WP_001265639.1	fimbrial adhesin EcpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	985310..1024047	38737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.89 Da
• Isoelectric Point: 8.2671

>T234662 WP_000854677.1 NZ_CP091712:995348-995716 [Escherichia coli]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T234662 NZ_LR134000:851440-851847 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11964.46 Da
• Isoelectric Point: 6.3163

>AT234662 WP_000065824.1 NZ_CP091712:995010-995327 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT234662 NZ_LR134000:851193-851459 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V0SXW1

Antitoxin

• AlphaFold DB: V0SU84