Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 285102..285599 Replicon chromosome
Accession NZ_CP091712
Organism Escherichia coli strain KFu014

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag L6L57_RS01330 Protein ID WP_001173343.1
Coordinates 285426..285599 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag L6L57_RS01325 Protein ID WP_000398619.1
Coordinates 285102..285398 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L57_RS01305 (281831) 281831..282550 - 720 WP_000611288.1 two-component system response regulator DcuR -
L6L57_RS01310 (282547) 282547..284178 - 1632 WP_001216471.1 sensor histidine kinase -
L6L57_RS01315 (284359) 284359..284589 + 231 WP_000371713.1 4Fe-4S mono-cluster protein YjdI -
L6L57_RS01320 (284601) 284601..284873 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
L6L57_RS01325 (285102) 285102..285398 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
L6L57_RS01330 (285426) 285426..285599 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
L6L57_RS01335 (285718) 285718..287235 - 1518 WP_000003799.1 lysine--tRNA ligase -
L6L57_RS01340 (287472) 287472..288929 - 1458 WP_000856843.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T234658 WP_001173343.1 NZ_CP091712:285426-285599 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T234658 NZ_LR133996:3143021-3143374 [Shimwellia blattae]
ATGTGGACTGTATTTTTTGGGCCAGTTTTTGATGCCTGGTTTGAAGAGCAAGAGCCCGCACTGAAGGAAAAAGTGTTAGC
CAACCTGCGCAATCTGGAAAAGTACGGTCCTTCTCTTTCCCGGCCATATGCGGATACCGTTTATGGCTCACGATATAAAA
ACATGAAGGAGTTGCGCATTCAGTATTCCGGATACCCTGTCCGGGCATTTTTTGCGTTTGATCCGGTTCGCCGTGCCATT
GTGCTTTGCGCCGGTGATAAAAGTAATGACAAAAAGTTTTACGACAGAATGATCCGCATTGCTGACGACGAGTTTTCCGC
TCACCTTGCAACGTTGGAGGGTAGTACGAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT234658 WP_000398619.1 NZ_CP091712:285102-285398 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT234658 NZ_LR133996:3143371-3143670 [Shimwellia blattae]
ATGAGAACACTAAAAGAAGTCATTGCCGCCCAGACGCCTGAGAGCCAGGCCCGCATTAACGAAATGGCCGAAGAGATGAT
TTTAGAAACGGGCCTCCAGATGATGCGTGAGGAGCTGCAGCTATCCCAGAAGGCGGTGGCCGACGCAATGGGCATCCGGC
AGCCTGCCATTACACAGATTGAACAACGCGGGAATGATATCAAACTGGCCACGCTCAAACGCTATATTGAGGCCATGGGG
GGTAAACTGAGCCTTGATGTTGAGTTGCCGACCGGTAAACGGATAGCTTTTCACATCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References