Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 83435..83960 Replicon plasmid pKGu016_1
Accession NZ_CP091705
Organism Escherichia coli strain KGu016

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag L6L59_RS25115 Protein ID WP_001159868.1
Coordinates 83435..83740 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag L6L59_RS25120 Protein ID WP_000813634.1
Coordinates 83742..83960 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L59_RS25100 (79369) 79369..80535 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
L6L59_RS25105 (81123) 81123..81878 - 756 WP_000852146.1 replication initiation protein RepE -
L6L59_RS25110 (82652) 82652..83434 - 783 WP_072644413.1 site-specific integrase -
L6L59_RS25115 (83435) 83435..83740 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
L6L59_RS25120 (83742) 83742..83960 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
L6L59_RS25125 (84668) 84668..85663 + 996 WP_000246635.1 hypothetical protein -
L6L59_RS25130 (85667) 85667..86599 + 933 WP_000991831.1 S-4TM family putative pore-forming effector -
L6L59_RS25135 (86897) 86897..87985 + 1089 WP_000952231.1 hypothetical protein -
L6L59_RS25140 (87978) 87978..88856 + 879 WP_225522546.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) - 1..100940 100940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T234651 WP_001159868.1 NZ_CP091705:c83740-83435 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T234651 NZ_LR133996:1491319-1491606 [Shimwellia blattae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAGTGGCGAAAGCTGGGCTCGACAGTACGTGAACAGTTTAA
AAAGAAGCTGGCTGAAGTGCTTGAGTCACCCCGAATTGAAGCGAACAAACTTCGCGGTATGCCTGATTGTTACAAGATAA
AGCTCAGGTCTTCAGGTTATCGTCTTGTCTATCAGGTCATTGACGCGCAGGTTGTCGTCTTTGTGATTTCTGTCGGGAAA
AGAGAACGCCCGGAAGTTTATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT234651 WP_000813634.1 NZ_CP091705:c83960-83742 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT234651 NZ_LR133996:1491080-1491319 [Shimwellia blattae]
ATGGGTAGCATTAACCTGCGTATTGATGATGAACTTAAAGCTCGCTCTTACGCTGCGCTTGAAAAAATGGGTATAACGCC
CTCTGAAGCGCTTCGTCTCATGCTTGAATATATCGCTGATAATGAACGTCTGCCGTTCAGACAGACACTCCTGAGTGATG
AAGATGCTGAGCTCGTGGAGATAATAAAAGAGCGTCTTCGCAATCCGAAGCCGGTCCGTGTGACGCTGGATGAGCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References