Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5251..5810 Replicon plasmid pKGu016_1
Accession NZ_CP091705
Organism Escherichia coli strain KGu016

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag L6L59_RS24655 Protein ID WP_000421272.1
Coordinates 5251..5526 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag L6L59_RS24660 Protein ID WP_001387467.1
Coordinates 5526..5810 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L59_RS24625 (1301) 1301..2041 - 741 WP_016238487.1 tyrosine-type recombinase/integrase -
L6L59_RS24630 (2411) 2411..2700 - 290 Protein_2 type II toxin-antitoxin system RelE/ParE family toxin -
L6L59_RS24635 (2687) 2687..2955 - 269 Protein_3 type II toxin-antitoxin system ParD family antitoxin -
L6L59_RS24640 (3487) 3487..4656 + 1170 WP_001072355.1 hypothetical protein -
L6L59_RS24645 (4689) 4689..4817 + 129 Protein_5 IS481 family transposase -
L6L59_RS24650 (4942) 4942..5145 - 204 Protein_6 hypothetical protein -
L6L59_RS24655 (5251) 5251..5526 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L6L59_RS24660 (5526) 5526..5810 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
L6L59_RS24665 (6415) 6415..7167 + 753 WP_000562172.1 IclR family transcriptional regulator -
L6L59_RS24670 (7213) 7213..8178 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
L6L59_RS24675 (8211) 8211..8591 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
L6L59_RS24680 (8616) 8616..9506 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
L6L59_RS24685 (9739) 9739..9933 - 195 WP_000796505.1 hypothetical protein -
L6L59_RS24690 (9907) 9907..10347 + 441 Protein_14 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) - 1..100940 100940


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T234646 WP_000421272.1 NZ_CP091705:c5526-5251 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T234646 NZ_LR133996:c56366-56145 [Shimwellia blattae]
GTGCATTACTGTATTCATACGACGCTCGCTCGTAAGCTCGACATCATCAATGCTGCCGTGACATATCTGGATTTAAGATC
GCCTCCAGGCAACAGATATGAAGAGCTAAACGGCAAACTGGAAGGTTATTCGTCAATTCGTATTAACCAGCAGTATCGCC
TGATATTTAAGTGGGTGAACGGAAAAGCAGAAGACCTCTACCTGGATCCGCATAAATACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT234646 WP_001387467.1 NZ_CP091705:c5810-5526 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT234646 NZ_LR133996:c56087-55752 [Shimwellia blattae]
ATGAAACAGGCAACCAGAAAACCAACTACTGTAGGTGACATCCTGCTGTATGAATACCTGGAGCCACTGGACCTTAAAAT
CAACGAACTTGCACAAATGTTACATGTGCATCGTAATAGCGTAAGTGCACTGGTTAATAACAACCGCAAACTCACGACTG
ACATGGCATTTCGCCTTGCCAAAGCCTTCGATACCTCCGTTGATTTCTGGCTTAACCTTCAGGCCGCTGTCGATCTGTGG
GAGGTAGAAAACGATCCCCGCGCGCAGGAAGAGCTCAGCCGCATCGTTCCCGTCGCCAGATTTATCGCCAGCAAAGCTGA
TAAGAAAATCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References